JH Regulation of a Hexamerin Gene Prior to Metamorphic Commitment

在昆虫的变态过程中，幼体激素调节基因表达的变化，这些变化对于昆虫身体形态的适当变化是必要的。本研究将分析在一个变态相关基因上操作的转录因子，该基因可转导幼年激素调节信号。这些蛋白质将被纯化，并通过荧光配体结合技术评估其配体结合特性。在体外转录分析中分析它们的配体结合和反激活特性，将为幼体激素调节与变态相关的基因表达的分子机制提供重要信息。

9818433JonesDuring insect metamorphosis, juvenile hormone regulates changes in gene expression that are necessary for properly executed changes in insect body form. This research will analyze the transcription factors operating at a metamorphosis-associated gene that transduce the juvenile hormone regulatory signals. These proteins will be purified and their ligand binding properties assessed by fluorescence ligand-binding techniques. Analysis of their ligand-binding and transactivation properties in the in vitro transcription assays will provide important information on the molecular mechanisms by which juvenile hormone regulates expression of this, and possibly other, genes associated with metamorphosis.