JH Regulation of a Hexamerin Gene Prior to Metamorphic Commitment

变态前六聚蛋白基因的 JH 调控

• 批准号: 9818433
• 负责人: Grace Jones
• 金额: $ 33万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9818433&HistoricalAwards=false
• 关键词: JH; Regulation; Hexamerin; Gene; Prior

9818433JonesDuring insect metamorphosis, juvenile hormone regulates changes in gene expression that are necessary for properly executed changes in insect body form. This research will analyze the transcription factors operating at a metamorphosis-associated gene that transduce the juvenile hormone regulatory signals. These proteins will be purified and their ligand binding properties assessed by fluorescence ligand-binding techniques. Analysis of their ligand-binding and transactivation properties in the in vitro transcription assays will provide important information on the molecular mechanisms by which juvenile hormone regulates expression of this, and possibly other, genes associated with metamorphosis.

9818433在昆虫变态过程中，保幼激素调节基因表达的变化，这些变化是昆虫体型正确变化所必需的。 本研究将分析在一个变态相关基因上运作的转录因子，该基因抑制保幼激素调节信号。 这些蛋白质将被纯化，并通过荧光配体结合技术评估其配体结合特性。 在体外转录试验中分析它们的配体结合和反式激活特性将提供保幼激素调节这种和可能的其他与变态相关的基因表达的分子机制的重要信息。