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Analytical Separations of Large Biopolymers through Capillary Electrophoresis

通过毛细管电泳分析分离大型生物聚合物

基本信息

批准号：
9321431
负责人：
Milos Novotny
金额：
$ 40.43万
依托单位：
Indiana University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1994
资助国家：
美国
起止时间：
1994-05-01 至 1997-10-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9321431&HistoricalAwards=false
关键词：
Analytical Separations Large Biopolymers through

项目摘要

This grant from the Analytical and Surface Chemistry Program will support Professor Novotny's investigations of the applications of pulsed-field capillary electrophoresis (CE) in the separation and characterization of polysaccharides and proteoglycans. The goal is to extend CE technology to high molecular weight biopolymers in the range of 100,000 to 100,000,000 Daltons. The materials to be investigated will include large proteins, DNA molecules with unusual shapes, polysaccharides, proteoglycans, and other biomolecules. In addition, a fundamental investigation of the factors which control the rate of migration of large molecules in pulsed electric fields will be carried out by Professor Novotny and his students. This research will extend the capabilities of capillary electrophoresis, a method that allows the separation of charged components present in complex macromolecular mixtures. The goal is to be able to separate large biomolecules with molecular weights up to 100,000,000 Daltons. Pulsed electric fields will be investigated in order to overcome many of the problems encountered in the fractionation of mixtures of large molecules by conventional capillary electrophoresis methods. The materials to be investigated include polysaccharides, carbohydrates, and proteoglycans. The techniques developed in this project will be used to separate large molecules of biochemical and technological significance

这笔来自分析和表面化学项目的资助将支持Novotny教授对脉冲场毛细管电泳（CE）在多糖和蛋白聚糖分离和表征中的应用的研究。目标是将CE技术扩展到100,000至100,000,000道尔顿范围内的高分子量生物聚合物。要研究的材料将包括大蛋白质、形状不寻常的DNA分子、多糖、蛋白多糖和其他生物分子。此外，诺沃特尼教授和他的学生将对控制脉冲电场中大分子迁移速率的因素进行基础研究。这项研究将扩展毛细管电泳的能力，毛细管电泳是一种允许分离存在于复杂大分子混合物中的带电成分的方法。目标是能够分离分子量高达1亿道尔顿的大型生物分子。为了克服传统毛细管电泳方法在大分子混合物分馏中遇到的许多问题，将研究脉冲电场。要研究的材料包括多糖、碳水化合物和蛋白多糖。本项目开发的技术将用于分离具有生物化学和技术意义的大分子