Development of a Project Based Laboratory Sequence In Molecular Biology And Chemistry

分子生物学和生物化学的一个基于项目的实验课程正在实施。新课程为学生提供连贯的、以项目为导向的实验室体验，使他们能够参与实验的选择和设计。实验课程使学生参与科学过程，从而促进科学家所依赖的反思判断和解决问题能力的发展。学生们正在熟悉分子生物学和生物化学最常用的技术来解决研究问题。在分子生物学课程中，引入了分子克隆和聚合酶链反应技术，然后学生使用DNA热循环器应用这些技术，并探索感兴趣的问题。在生物化学课程中，蛋白质的纯化是通过使用重组DNA技术而不是依靠传统的工具来实现的。生物化学课程也使用高效液相色谱法来实现生物分子的困难分离。

Development of a Project Based Laboratory Sequence in Molecular Biology and Biochemistry A project based laboratory curriculum in Molecular Biology and Biochemistry is being implemented. The new curriculum offers students a coherent, project oriented laboratory experience that allows them to participate in the selection and design of experiments. The laboratory curriculum is engaging students in the scientific process and thereby is facilitating the development of the reflective judgment and problem solving skills relied upon by scientists. The students are being acquainted with the techniques of molecular biology and biochemistry most commonly used to address research questions. In the Molecular Biology course, both molecular cloning and polymerase chain reaction techniques are being introduced, and students then are applying these techniques, using the DNA thermocycler, and exploring a question of interest. In the Biochemistry course, protein purification is being approached by using recombinant DNA technology rather than by relying upon classical tools. The Biochemistry course also is using high performance liquid chromatography to effect difficult separations of biomolecules.