Dissertation Research: Experimental Molecular Evolution in Bacteriophage

论文研究：噬菌体的实验分子进化

• 批准号: 9411950
• 负责人: James Bull
• 金额: $ 0.75万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-01 至 1997-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9411950&HistoricalAwards=false
• 关键词: Dissertation; Research; Experimental; Molecular; Evolution

9411950 Bull This experimental study examines molecular evolution in recombinant DNA phages. The recombinant phage are constructed to specifically address two topics of interest in molecular evolution: 1) the molecular evolution of essential transgenes (phage capsid and assembly genes) and 2) the evolution of RNA polymerase-promoter interactions. Transgenic phage will be characterized at the molecular level via sequence analysis and at the phenotypic level via fitness levels. The fitness of transgenic phage is initially low, but increases rapidly when subjected to laboratory natural selections via serial propagation. After a response to selection for increased fitness is observed, the phage will be re- characterized at the molecular and phenotypic levels. Initial genetic manipulations will lead to specific expectations as to which genetic elements should evolve and how they will change over the course of evolution. This system of experimental molecular evolution in bacteriophage is a powerful new approach in evolutionary biology. Transgenic organisms have become increasingly popular in many areas of biology and industry. This project will for the first time directly address questions concerning how transgenes evolve, what their effects are on other genes, and how these combined molecular changes affect evolution of fitness at the organismal level.

小行星9411950 本实验研究探讨了重组DNA测序中的分子进化。 构建重组噬菌体以专门解决分子进化中的两个感兴趣的主题：1）必需转基因（噬菌体衣壳和组装基因）的分子进化和2）RNA聚合酶-启动子相互作用的进化。 转基因噬菌体将通过序列分析在分子水平上表征，并通过适合度水平在表型水平上表征。 转基因噬菌体的适应性最初较低，但当通过连续繁殖进行实验室自然选择时迅速增加。 在观察到对增加的适合度的选择的响应后，噬菌体将在分子和表型水平上重新表征。 最初的基因操作将导致特定的期望，即哪些基因元件应该进化，以及它们在进化过程中将如何变化。 这种噬菌体分子进化实验系统是进化生物学中一种强有力的新方法。 转基因生物在生物学和工业的许多领域越来越受欢迎。 该项目将首次直接解决有关转基因如何进化的问题，它们对其他基因的影响，以及这些组合的分子变化如何影响生物体水平上的适应性进化。