Acquisition of Protein Chemistry Instrumentation

本提案中讨论的项目代表了爱荷华州州立大学化学系和其他系的跨学科工作，涉及一个共同的主题：蛋白质结构和动力学对蛋白质功能的重要性。 在这个建议中讨论的问题范围从组装和折叠的蛋白质，蛋白质的结构作用，在确定天然存在的光合色素之间的有效能量转移，以及蛋白质与表面相互作用的方式。 为了解决这些典型问题，需要专用设备。 由于蛋白质是提案的中心主题，因此需要大规模合成和纯化长链肽并表征其结构完整性的仪器。 将对精心设计的蛋白质进行文中所述的研究，这些研究通过傅里叶变换红外和拉曼技术表征它们与表面的相互作用，或者通过各种时间分辨技术在从数百飞秒到数百纳秒的时间尺度上监测它们的反应性。

The projects discussed in this proposal represent interdisciplinary work in the Department of Chemistry and other Departments at Iowa State University dealing with a common theme: the importance of protein structure and dynamics for protein function. The problem discussed in this proposal range from the assembly and folding of proteins, the structural role of proteins in determining efficient energy transfer among naturally occurring photosynthetic pigments, and the way in which proteins interact with surfaces. In order to address these representative problems, dedicated equipment is required. Because proteins are the central theme of proposal, instruments to synthesize and to purify long-chain peptides on a large scale and to characterize their structural integrity are requested. The carefully designed proteins will be subjected to studies described in the text that characterize their interactions with surfaces either by Fourier transform infrared and Raman techniques or that monitor their reactivity on time scales from hundreds of femotseconds to hundreds of nanoseconds by various time- resolved techniques.