Acquisition of Protein Chemistry Instrumentation

收购蛋白质化学仪器

• 批准号: 9413969
• 负责人: Jacob Petrich
• 金额: $ 32.5万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-15 至 1997-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9413969&HistoricalAwards=false
• 关键词: Acquisition; Protein; Chemistry; Instrumentation

The projects discussed in this proposal represent interdisciplinary work in the Department of Chemistry and other Departments at Iowa State University dealing with a common theme: the importance of protein structure and dynamics for protein function. The problem discussed in this proposal range from the assembly and folding of proteins, the structural role of proteins in determining efficient energy transfer among naturally occurring photosynthetic pigments, and the way in which proteins interact with surfaces. In order to address these representative problems, dedicated equipment is required. Because proteins are the central theme of proposal, instruments to synthesize and to purify long-chain peptides on a large scale and to characterize their structural integrity are requested. The carefully designed proteins will be subjected to studies described in the text that characterize their interactions with surfaces either by Fourier transform infrared and Raman techniques or that monitor their reactivity on time scales from hundreds of femotseconds to hundreds of nanoseconds by various time- resolved techniques.

本提案中讨论的项目代表了爱荷华州立大学化学系和其他系的跨学科工作，涉及一个共同主题：蛋白质结构和动力学对蛋白质功能的重要性。 该提案讨论的问题包括蛋白质的组装和折叠、蛋白质在确定天然存在的光合色素之间的有效能量转移中的结构作用，以及蛋白质与表面相互作用的方式。 为了解决这些代表性问题，需要专用设备。 由于蛋白质是提案的中心主题，因此需要大规模合成和纯化长链肽并表征其结构完整性的仪器。 精心设计的蛋白质将接受文本中描述的研究，通过傅里叶变换红外和拉曼技术来表征它们与表面的相互作用，或者通过各种时间分辨技术在数百飞秒到数百纳秒的时间尺度上监测它们的反应性。