Instruments for Fluorescence and Video-Enhanced Microscopy

荧光和视频增强显微镜仪器

• 批准号: 9418205
• 负责人: Wendy Boss
• 金额: $ 4.79万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9418205&HistoricalAwards=false
• 关键词: Instruments; Fluorescence; Video; Enhanced; Microscopy

This proposal requests funds for equipment for fluorescence and video-enhanced microscopy to include a cooled charged coupled device (CCD) camera and imaging system for detecting low intensity fluorescence, a fluorescence microscope equipped with infinity corrected and differential interference contrast optics, an image processing system and Newvicon camera for video-enhanced microscopy, and a micromanipulator and piezoelectric microinjector for use with plant cells. This is a joint proposal by 7 investigator (5 major users, Wendy F. Boss, Rebecca S. Boston, Mark Conkling, Dominique Robertson, Ron Sederoff and Ross Whetten, and 2 minor users, Tim Petty and Kelly Tatchell) from four departments and two colleges. To our knowledge there is no other cooled CCD camera on campus and there is no comparable equipment for high resolution video-enhanced microscopy in the College of Agriculture and Life Sciences or the College of Forestry Resources. The principle investigators study basic cellular processes and signal transduction using a combination of molecular, genetic, biochemical, and microscopic techniques. The research projects include signal transduction (Boss), protein trafficking and packaging to storage vesicles in maize (Boston), root-specific gene expression and nematode resistance (Conkling), viral pathogenesis (Robertson and Petty), cell division and cell cycle control (Tatchell), and lignin biosynthesis, fungal disease resistance and genetic mapping in loblolly pine (Sederoff and Whetten). For the above studies we need to visualize fluorescent proteins within living cells, to monitor changes in protein distribution and cellular morphology over time, and to resolve fluorescent markers on metaphase chromosomes. Our research is currently limited by the sensitivity and resolution of existing microscopic equipment. The Botany Department has made a commitment to the facility by providing space and technical support. The equipment will be under the s upervision of Dr. Wendy F. Boss and Mr. Dana Moxley, and it will be housed in a central location. The requested equipment will contribute significantly to our ongoing efforts to strengthen cell biology at North Carolina State University.

本提案要求提供资金，用于购置荧光和视频增强显微镜设备，包括一台用于探测低强度荧光的冷却式电荷耦合器件照相机和成像系统、一台配备无限校正和微分干涉对比光学器件的荧光显微镜、一台图像处理系统和用于视频增强显微镜的Newvicon照相机，以及用于植物细胞的显微操作器和压电显微注射器。 这是一项由7名研究人员（5名主要用户，Wendy F.头儿，丽贝卡·S。波士顿，马克康克林，多米尼克罗伯逊，罗恩塞德罗夫和罗斯惠顿，和2个小用户，蒂姆佩蒂和凯利塔蒂）从四个部门和两个学院。 据我们所知，校园里没有其他冷却CCD相机，农业和生命科学学院或林业资源学院也没有类似的高分辨率视频增强显微镜设备。 主要研究人员使用分子，遗传，生物化学和显微技术的组合研究基本的细胞过程和信号转导。 研究项目包括信号转导（Boss）、蛋白质运输和包装到玉米（Boston）中的储存囊泡、根特异性基因表达和线虫抗性（Conkling）、病毒发病机理（Robertson和Petty）、细胞分裂和细胞周期控制（Tatterus）以及火炬松（Sederoff和Whetten）中的木质素生物合成、真菌病害抗性和遗传图谱。 对于上述研究，我们需要可视化活细胞内的荧光蛋白，以监测蛋白质分布和细胞形态随时间的变化，并解决中期染色体上的荧光标记。 我们的研究目前受到现有显微设备的灵敏度和分辨率的限制。 植物学部承诺为该设施提供空间和技术支持。 该设备将由Wendy F博士监督。老板和戴娜·莫克斯利先生，它将被安置在一个中心位置。 所要求的设备将大大有助于我们正在进行的努力，以加强细胞生物学在北卡罗来纳州州立大学。