Acquisition of an Image Plate Area Detector for X-Ray Crystallography

用于 X 射线晶体学的图像板区域探测器的采集

• 批准号: 9420048
• 负责人: Thomas Alber
• 金额: $ 22.34万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-01 至 1997-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9420048&HistoricalAwards=false
• 关键词: Acquisition; Image; Plate; Area; Detector

Funds are requested for an X-ray data collection system for macromolecular crystal structure determination. The instrument is the R-AXIS II imaging plate system, which includes a rotating anode X-ray generator, focusing mirrors, and a controlling computer. The new instrument will be located in Stanley Hall at the University of California, Berkeley, and will be shared by three research groups which- use X-ray crystallography as a primary research tool. Currently, the three research groups share one X-ray area detector, which is used full time, and the backlog of data collection limits progress. The three groups have a broad range of research interests. The proteins and peptides under investigation differ significantly in size and biological function. Prof. Alber asks general questions about protein folding, stability, and intra- and intermolecular protein interactions. Model systems used to test these questions include coiled-coils, T4 lysozyme, ATCase, and transcriptional accessory proteins. Prof. Nelson studies DNA binding proteins and protein-DNA interactions. Crystals of the DNA binding domain of the heat shock transcription factor diffract X-rays to at least 0.9 resolution, making this the highest resolution structure for which mutants are readily obtained. Prof. Stevens studies proteins involved in neurotransmission. Proteins under study include: two proteins involved in catecholamine biosynthesis, tyrosine hydroxylase and dopamine monoxygenase; neuroreceptors, such as human 2-adrenergic receptor, fish olfactory receptor, and yeast factor receptor; and the botulinum neurotoxin. In addition, to the individual research interests of the three groups, collaborative crystallographic projects have been initiated with other faculty at Berkeley. Clearly, an additional instrument will allow significant progress to made on a wide range of crystallographic studies. In addition to supporting the research efforts of these three groups, this instrument will have a train ing function. Between the three groups, there are 4 post-doctoral fellows, 14 graduate students, and 4 undergraduates who are pursuing crystallographic projects. The size of the joint group is expected to grow by 6 more students and fellows within the next year.

要求提供资金，用于建立一个X射线数据收集系统，以确定大分子晶体结构。该仪器是R-AXIS II成像板系统，包括旋转阳极X射线发生器，聚焦镜和控制计算机。新仪器将位于加州大学伯克利分校的斯坦利大厅，并将由三个研究小组共享-使用X射线晶体学作为主要研究工具。目前，三个研究小组共用一个全时使用的X射线区域探测器，数据收集的积压限制了进展。 这三个小组有广泛的研究兴趣。所研究的蛋白质和肽在大小和生物学功能上有显著差异。阿尔伯教授询问有关蛋白质折叠，稳定性，以及分子内和分子间蛋白质相互作用的一般问题。 用于测试这些问题的模型系统包括卷曲螺旋，T4溶菌酶，ATCase和转录辅助蛋白。纳尔逊教授研究DNA结合蛋白和蛋白质-DNA相互作用。热休克转录因子的DNA结合结构域的晶体可将X射线衍射到至少0.9的分辨率，使其成为容易获得突变体的最高分辨率结构。史蒂文斯研究参与神经传递的蛋白质。正在研究的蛋白质包括：参与儿茶酚胺生物合成的两种蛋白质，酪氨酸羟化酶和多巴胺单加氧酶;神经受体，如人β 2-肾上腺素能受体、鱼嗅觉受体和酵母因子受体;以及肉毒杆菌神经毒素。此外，三个小组的个人研究兴趣，合作结晶学项目已开始与伯克利分校的其他教师。显然，一个额外的仪器将允许在广泛的晶体学研究方面取得重大进展。 除了支持这三个小组的研究工作外，该仪器还具有培训功能。在这三个小组中，有4名博士后研究员，14名研究生和4名正在进行晶体学项目的本科生。联合小组的规模预计将在明年增加6名学生和研究员。