Acquisition of Equipment for Automated DNA Sequencing

购置自动 DNA 测序设备

• 批准号: 9512178
• 负责人: Lynn Jorde
• 金额: $ 11.29万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-08-15 至 1996-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9512178&HistoricalAwards=false
• 关键词: Acquisition; Equipment; Automated; DNA; Sequencing

With partial National Science Foundation support, the University of Utah will purchase an ABI 3377 automated DNA sequencer, an Perkin-Elmer 9600 PCR machine and a Sun SPARCstatioon 20. This equipment will be shared by four evolutionary geneticists. Acquisition of this equipment will greatly increase the speed and fidelity of DNA sequencing and nuclear genotyping and enable the scientists to remain at the cutting edge of human evolutionary genetic research. Initially, the equipment will be used in at least 13 major research projects. These include research on the evolutionary genetics of south Indian caste populations, Micronesian populations, the Altai Siberian population, Native American populations and African and African-American populations. To provide a long-term temporal perspective, ancient DNA samples from the U.S. Southwest, the Great Basin region, North China, the circum-Arctic, and Austro-Hungary will be analyzed. The role of founder effect in the evolution of von Willebrand disease will be investigated. Mutation rates in mitochondrial DNA will be measured by comparing large-scale sequencing results with `DNA chip` technology. Worldwide genetic variation will be analyzed to test hypotheses regarding the origins of modern Homo sapiens. In addition to aiding in research, this equipment will help students and postdoctoral fellows to learn the most modern molecular methods in the course of their training in evolutionary genetics. Each of the researchers' laboratories has a number of students and fellows.

在国家科学基金会的部分支持下，犹他州大学将购买ABI 3377自动DNA测序仪、Perkin-Elmer 9600 PCR仪和Sun SPARCstatioon 20。这套装备将由四位进化基因学家共享。获得这种设备将大大提高DNA测序和核基因分型的速度和保真度，使科学家能够保持在人类进化遗传学研究的前沿。 最初，这些设备将用于至少13个重大研究项目。其中包括对南印度种姓人口、西伯利亚人口、阿尔泰西伯利亚人口、美洲土著人口以及非洲人和非裔美国人人口的进化遗传学研究。为了提供一个长期的时间视角，来自美国西南部，大盆地地区，中国北方，环北极和奥匈帝国的古代DNA样本将被分析。奠基者效应在血管性血友病演变中的作用将被研究。线粒体DNA的突变率将通过将大规模测序结果与“DNA芯片”技术进行比较来测量。世界范围内的遗传变异将被分析，以测试关于现代智人起源的假设。 除了协助研究外，该设备还将帮助学生和博士后研究员在进化遗传学培训过程中学习最现代的分子方法。每个研究人员的实验室都有一些学生和研究员。