RUI: Acquisition of Automated DNA Sequencing Equipment for East Carolina University

RUI：为东卡罗来纳大学采购自动 DNA 测序设备

• 批准号: 0071384
• 负责人: Edmund Stellwag
• 金额: $ 13.29万
• 依托单位: East Carolina University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0071384&HistoricalAwards=false
• 关键词: RUI; Acquisition; Automated; DNA; Sequencing

RUI: Acquisition of Automated DNA Sequencing Equipment for East Carolina University. This award, combined with matching funds from East Carolina University, will be used to purchase an Applied Biosystems 377 automated DNA sequencer, a Silicon Graphics 2000 parallel processing computer system, a gradient programmable thermal cycler and an automated plasmid DNA purification system for revitalization and expansion of the centralized DNA sequencing facility. Seven principal and several minor investigators, as well as their students, will use the centralized facility for sequencing intensive research covering diverse aspects of the biological sciences. In specific, this DNA sequencing intensive research includes molecular phylogenetics and biogeography, character evolution, evolution and function of the teleost immune system, vertebrate evolutionary developmental biology, organellar-nuclear genome gene regulation, gene regulation of stress response in plants and structural organization of photosynthetic membrane protein complexes. The Department of Biology at East Carolina has a strong research program that places a heavy emphasis on training undergraduate and Master's level graduate students in research. The department offers Bachelor's degrees in Biology and Biochemistry and Master's degrees in Biology and Molecular Biology and Biotechnology. The number of undergraduates electing the molecular biology and biotechnology areas of concentration and the number of Master's degrees in Molecular Genetic and Biotechnology has increased significantly. The University is adding courses in bioinformatics, molecular biology, molecular genetics, and protein purification and structure analysis. This award will facilitate these course offerings by allowing the Center to provide access to state-of-the-art equipment in these courses.

RUI：为东卡罗莱纳大学购置自动DNA测序设备。这笔奖金加上东卡罗莱纳大学的配套资金，将用于购买Applied Biosystems 377自动DNA测序仪、Silicon Graphics 2000并行处理计算机系统、梯度可编程热循环仪和自动质粒DNA纯化系统，以振兴和扩大集中式DNA测序设施。 七名主要研究人员和几名次要研究人员以及他们的学生将使用集中设施进行测序密集研究，涵盖生物科学的各个方面。具体而言，该DNA测序密集型研究包括分子遗传学和植物地理学，性状进化，硬骨鱼免疫系统的进化和功能，脊椎动物进化发育生物学，细胞器-核基因组基因调控，植物胁迫反应的基因调控和光合膜蛋白复合物的结构组织。生物学系在东卡罗莱纳有一个强大的研究计划，把重点放在培养本科和硕士研究生水平的研究。该系提供生物学和生物化学学士学位以及生物学和分子生物学和生物技术硕士学位。 选修分子生物学和生物技术专业的本科生人数以及攻读分子遗传学和生物技术硕士学位的人数大幅增加。 该大学正在增加生物信息学、分子生物学、分子遗传学以及蛋白质纯化和结构分析等课程。 该奖项将通过允许中心在这些课程中提供最先进的设备来促进这些课程的提供。