A Tabletop Ultracentrifuge for Membrane and Nucleic Acid Preparations

提交该提案是为了资助购买台式超速离心机。这种类型的离心机特别适用于细胞级分的分离和核酸样品的纯化，其中需要小体积、高速和短旋转时间。该仪器将用于三个主要项目： (1)膜制备：细菌膜部分的分离和重建将在大肠杆菌孔蛋白和其它细菌离子通道的电生理学研究的背景下进行。 (2)在非洲爪蟾胚胎神经诱导过程中基因调控研究的背景下，将通过铯梯度离心从胚胎外植体中分离RNA。 (3)RNA将通过铯梯度离心从斑马鱼松果体中分离，用于光和生物钟的基因调控研究 我们的研究工作的生产力和成功将大大提高收购拟议的仪器，这将使我们能够执行程序，是不可行的与我们的仪器的现状。

This proposal is submitted for funding the purchase of a tabletop ultracentrifuge. This type of centrifuge is particularly useful for the isolation of cellular fractions and purification of nucleic acid samples, where small volumes, high speed and short spin times are required. The instrument will be used in three major projects: (1) Membrane preparation: isolation and reconstitution of bacterial membrane fractions will be performed in the context of the electrophysiological study of Escherichia coli porins and other bacterial ion channels. (2) Isolation of RNA by cesium gradient centrifugation will be performed from embryonic explants in the context of the study of gene regulation during neural induction of Xenopus embryos. (3) RNA will be isolated by cesium gradient centrifugation from zebrafish pineal glands in studies of gene regulation by light and the circadian clock The productivity and success of our research efforts will be greatly enhanced by the acquisition of the proposed instrument, which will allow us to perform procedures that are not feasible with the present state of our instrumentation.