VPW: Development of New Techniques for Chromosome Mapping in Salmonid Fishes

本研究的目的是开发鲑科鱼类染色体定位的新方法，包括使用SINE序列进行染色体鉴定的条带技术，物种特异性和染色体特异性油漆探针，以及使用cosmid克隆进行基因定位的原位杂交技术。虽然在鲑鱼中有广泛的等位酶位点连锁图谱，但没有单一拷贝基因被映射到特定的染色体上。染色体图谱的构建将极大地促进渔业研究，并提高salamonfish作为发育遗传学、生态遗传学和毒理学研究模型的有效性。精确的地图也将使我们能够重建染色体进化。在我1995-1996学年访问华盛顿大学期间，将开展开发这些方法的工作。华盛顿大学非常适合这项研究，因为它的渔业学院拥有优秀的实验室来研究鲑科鱼类，而且分子生物学和生物技术系拥有染色体流动分选和单拷贝原位杂交的专业知识。菲利普斯博士正在与渔业学院的教职员工开展关于染色体定位和相关项目的合作项目。互动活动包括教授分子系统学的研究生研讨会，并在分子技术课上进行客座讲座。

The objective of this research is to develop new methods for chromosome mapping in salmonid fishes including banding techniques using SINE sequences for chromosome identification, species- specific and chromosome-specific paint probes, and in situ hybridization using cosmid clones for localization of genes. Although there is an extensive linkage map for allozyme loci in salmonids, no single copy genes have been mapped to specific chromosomes. Construction of chromosome maps would greatly benefit fisheries research and enhance the usefulness of salamonid fishes as models for research in developmental genetics, ecological genetics, and toxicology. Accurate maps will also allow us to reconstruct chromosome evolution. Work on development of these methods will be done during my visit to University of Washington in the 1995-1996 academic year. The University of Washington is uniquely suited for this research because the School of Fisheries maintains excellent laboratories for research on salmonid fishes and expertise in the flow sorting of chromosomes and single copy in situ hybridization exists in the department of Molecular Biology and Biotechnology. Dr. Phillips is developing collaborative projects with faculty members in the School of Fisheries on chromosome mapping and related projects. Interactive activities include teaching a graduate seminar in molecular systematics and giving guest lectures in the Molecular Techniques class.