A Consolidated and Enhanced Molecular Biology Facility for the Whitney Marine Laboratory

惠特尼海洋实验室的巩固和增强的分子生物学设施

• 批准号: 9602526
• 负责人: Peter A. V. Anderson
• 金额: --
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-10-01 至 2000-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9602526&HistoricalAwards=false
• 关键词: Consolidated; Enhanced; Molecular; Biology; Facility

The Whitney Marine Laboratory is a research institute of the University of Florida. It was established in 1974 and dedicated to a traditional role of marine laboratories; the use of marine animals as experimental models with which to address fundamental questions in cellular and neurobiology. This particular approach to marine biology has come to characterize the Laboratory within Florida, the Southeast region, and nationally. In 1989, the Laboratory initiated a program in molecular biology. This program proved to be enormously powerful and popular, so much so that nine of the twelve resident research groups at the Whitney Laboratory now use molecular cloning as a routine component of their research. To accommodate this growth, the Laboratory assigned additional space to molecular biology- the facilities currently consist of 21/2 functionally separate rooms, occupying a total of 630 ft2 , and a separate isotope lab. However, on given day, 12 individuals vie for bench space in any given room, and during peak times, visitors and students might raise this number to as many as 20. This heavy demand inevitably raises problems of scheduling and, most worrisome, contamination of cultures and PCR by extraneous DNA and RNA. Moreover, several essential items of equipment are already quite old, and, in many cases (e.g.,) the autoclave, shaking incubator and ice machine) they are the only item of their kind in the Laboratory, making the whole program is vulnerable to stoppage should one item break down. Finally, because the existing facilities are spread across four widely spaced rooms, the use of the facilities is inefficient. To correct these problems this project will support a consolidated molecular biology facility, organized and equipped so as to as to maximize its usage and utility to the resident faculty of the Whitney Laboratory and to visiting scientists and students. This consolidated facility would continue to operate under the skilled s upervision of the Laboratory's molecular biologist.

惠特尼海洋实验室是佛罗里达大学的一个研究机构。 它成立于1974年，致力于海洋实验室的传统作用;使用海洋动物作为实验模型，以解决细胞和神经生物学中的基本问题。 这种特殊的海洋生物学方法已经成为佛罗里达，东南地区和全国实验室的特点。 1989年，该实验室启动了一项分子生物学计划。 事实证明，该计划非常强大且受欢迎，以至于惠特尼实验室的12个常驻研究小组中有9个现在将分子克隆作为其研究的常规组成部分。 为了适应这一增长，实验室分配了额外的空间给分子生物学-设施目前包括21/2功能独立的房间，总占地630平方英尺，和一个单独的同位素实验室。 然而，在特定的一天，12个人在任何给定的房间里争夺长凳空间，在高峰期，游客和学生可能会将这个数字提高到20个。 这种巨大的需求不可避免地引起了时间安排的问题，最令人担忧的是，培养物和PCR被外来DNA和RNA污染。 此外，一些基本设备已经相当陈旧，在许多情况下（例如，）高压灭菌器、振荡培养箱和制冰机），它们是实验室中唯一的同类设备，如果其中一个设备出现故障，整个程序就很容易中断。 最后，由于现有的设施分布在四个间隔很大的房间里，设施的使用效率很低。 为了纠正这些问题，该项目将支持一个统一的分子生物学设施，组织和装备，以便最大限度地提高其使用和效用的惠特尼实验室的驻地教师和访问的科学家和学生。 这一综合设施将继续在实验室分子生物学家的熟练指导下运作。