A Microscope and Cryostat for the Whitney Laboratory

这是一个购买光学显微镜和 惠特尼医院组织切片用恒温器 佛罗里达大学海洋实验室。 这 设备特别适用于免疫细胞化学 发展研究， 分泌和信号传递。 这些研究 采用多种现代细胞和分子生物学 技术，但由于任何发现必须，最终， 根据细胞或细胞内 本地化的过程中的问题，使用 定位特定分子的技术已经成为 越来越重要。 这组调查人员 要求资金购买（1）一个新的低温恒温器，以取代 非常频繁使用但老化的单位，其操作非常 效率低，耗时，（2）研究等级 复合显微镜，迄今为止还没有得到在 实验室 这些单位将设在一个中央 组织学/显微镜设施，目前正在建设。

This is a proposal to purchase an optical microscope and a cryostat for histological sectioning for use at the Whitney Marine Laboratory of the University of Florida. This equipment is particularly intended for immunocytochemical studies of development, secretion and signaling in marine animals. These studies employ of a variety of modern cell and molecular biological techniques, but since any findings must, ultimately, be evaluated with respect to the cellular or intracellular localization of the processes in question, the use of techniques for localizing specific molecules has become increasingly important. This group of investigators requests funds to acquire (1) a new cryostat to replace a very heavily used but aging unit whose operation is very inefficient and time consuming and (2) a research grade compound microscope which hitherto has not been available at the laboratory. These units will be housed in a central histology/microscopy facility that is currently being built.