The Evolution of Calcium Channels

钙通道的演变

• 批准号: 9723438
• 负责人: Peter A. V. Anderson
• 金额: $ 4.79万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9723438&HistoricalAwards=false
• 关键词: Evolution; Calcium; Channels

Peter Anderson Subject: Re: IBN-9723438 ABSTRACT Voltage gated calcium channels are a family of proteins that are essential for many cell functions, including excitation, contraction, secretion, enzyme activity and gene regulation. They exist in living cells as multisubunit complexes, usually consisting of an alpha1 subunit and associated beta, gamma and alpha2/delta subunits. The pore forming alpha1 subunits, which are primarily responsible for many of the physiological and pharmacological properties of the channels, can be divided on their basis of their structure, into 6 separate classes. A full length calcium channel cDNA was recently cloned from the jellyfish Cyanea capillata, by our lab. The deduced amino acid sequence of this protein suggests that it is a member of the L-type family of calcium channels, and this is supported by much of its physiology and pharmacology. However, there are some significant deviations from the overall properties of calcium channels, in particular the ionic selectivity of the channel and its sensitivity to dihydropyridines, a class of calcium channel blockers that are diagnostics of L-type calcium channels. The aim of the proposed work is to complete the characterization of the jellyfish calcium channel with a view to using the physiological and pharmacological abnormalities of this channel as a means of identifying the structural correlates of L-type channel function.

彼得·安德森 主题：回复：IBN-9723438 电压门控性钙通道是一类在细胞兴奋、收缩、分泌、酶活性和基因调控等多种功能中发挥重要作用的蛋白质家族。 它们以多亚基复合物的形式存在于活细胞中，通常由α 1亚基和相关的β、γ和α 2/δ亚基组成。 主要负责通道的许多生理学和药理学性质的成孔α 1亚基可根据其结构分为6个单独的类别。 本实验室最近从水母Cyanea capillata中克隆了一个全长钙通道cDNA。 这种蛋白质的推导氨基酸序列表明它是L型钙通道家族的成员，这得到了其生理学和药理学的支持。 然而，与钙通道的总体特性存在一些显着偏差，特别是通道的离子选择性及其对二氢吡啶的敏感性，二氢吡啶是一类钙通道阻滞剂，是L型钙通道的诊断剂。拟议的工作的目的是完成水母钙通道的表征，以期使用该通道的生理和药理异常作为一种手段，确定L-型通道功能的结构相关性。