Membrane Lipid Biosynthesis in Chlamydomonas Reinhardtii

9603626某种形式的摩尔细胞分裂是所有生物繁殖的中心，也是更复杂的生命形式的生长和繁殖的核心。对这一划分的监管至关重要，而监管的丧失对所有形式的生命都会产生可怕的后果。正因为如此，人们对分工过程进行了广泛的研究，对许多方面的了解也越来越深入。该工艺的一个组成部分尚未得到充分研究，那就是对膜生产的控制。细胞膜包围着细胞的外围部分和细胞器，这些细胞器专门负责细胞的特定代谢功能。产生太多、太少、或膜的错误成分，或在错误的时间，都会导致子代细胞的异常。这项研究将致力于测量在细胞分裂周期中发生的膜成分的变化以及伴随这些变化的生物合成/分解代谢过程。将利用光合作用有机体莱茵衣藻，工作将主要集中在其主要的外质膜脂类，磷脂酰乙醇胺和二酰甘油三甲基高丝氨酸。由于这两种脂类的合成途径尚未确定，因此确定这两种脂类的合成途径和细胞内位置将是首要任务。该项目涉及与细胞分裂和细胞周期有关的膜的合成的调节，特别是其脂质成分的调节。如果膜合成与细胞分裂不适当地协调，可能会导致严重的问题。我们将研究一种可以通过基因操作的简单藻类--莱茵衣藻。将分析和研究两种主要脂质的合成途径。该项目将提供有关植物如何控制其生长和繁殖的新信息，并可能对作物生产产生长期影响。

9603626 Moore Cell division in some form is central to reproduction of all organisms, and also to growth and reproduction of more complex forms of life. Regulation of this division is crucial, and loss of regulation has dire consequences for all forms of life. Because of this, extensive studies of the division process have been conducted and many aspects are increasingly well understood. One component of the process which has not been sufficiently studied is the control over membrane production. Membranes surround both the periphery and the cellular components, organelles, which specialize in specific metabolic functions of the cell. Production of too much, too little, or the wrong components of the membranes, or at the wrong time, would lead to aberrations in the daughter cells. The research would be devoted to measuring the changes in membrane composition which takes place during a cell division cycle and the biosynthetic/catabolic processes which accompany these changes. The photosynthetic organism, Chlamydomonas reinhardtti, will be utilized and the work will concentrate primarily on its major extraplastid membrane lipids, phosphatidylethanolamine and diacylglyceroltrimethylhomoserine. Since the pathways of synthesis of these two lipids are not defined for C. reinhardtti, determination of the pathways and intracellular locations of synthesis of these two lipids will receive initial priority. This project is concerned with regulation of synthesis of membranes, particularly their lipid components, in relation to cell division and the cell cycle. If membrane synthesis is not properly coordinated with cell division, serious problems could result. A simple alga, Chlamydomonas reinhardtii, that can be manipulated genetically will be studied. The pathway for synthesis of two major lipids will be analyzed and studied. This project will provide new information about how plants control their growth and reproduction and may have a long-term impact on crop production.