A Preparative Ultracentrifuge and Rotors for Separation and Analysis of Biological Samples

请拨资金购买一个新的制备型超速离心机和三个超速离心机转子，供蒙大拿州立大学兽医分子生物学系的四个实验室使用。超速离心机是所有生物化学和分子生物学研究的重要设备。目前，该部只有一名22岁的贝克曼L5-65岁的超级离心机，不能满足该部目前和未来的需要。此外，L5正在迅速淘汰，新的驱动器将不再可用于维修这台离心机。由于去年(Quinn博士和Pascual博士均于1995年受聘)和来年(另一名教员将于明年加入该系)增加的教职员工增加了对超速离心法的需求，这进一步强调了我们对所需设备的需求。该部还缺乏足够的转子来开展拟议的研究，这项提案包括3个新的转子。目前，该部门有一个较老的固定角度转子(10岁)，一个摆动的斗形转子，和一个降级的50型转子。这些转子都不能以每分钟60,000转的速度运行。可能不应该使用超过45,000 r.pm。本提案中要求的旋翼能够有更高的速度，并将促进更快和更有效的分离。新设备将主要由Quinn博士、Jutila博士、Pascual博士和Burgess博士的实验室使用，因此本提案包含对他们利用超速离心法的项目的描述。这些项目都是由N1H、NSF和美国农业部等多个机构的研究经费资助的。超速离心是这些项目的一部分，用于蛋白质和蛋白质复合体的密度梯度分析、亚细胞分离、膜纯化和DNA纯化。此外，还将有少量的离心机时间(5%)用于部门班级，培训学生使用现代生物仪器。

Funds are requested to purchase a new preparative ultracentrifuge and three ultracentrifuge rotors which will be used by four laboratories in the Department of Veterinary Molecular Biology at Montana State University. Ultracentrifuges are essential equipment for all research in biochemistry and molecular biology. Currently, there is only one ultracentrifuge in this Department, a 22 year old Beckman L5-65, which is not capable of meeting the Department's current and future needs. In addition, the L5 is rapidly becoming obsolete, and new drives will no longer be available for repairing this centrifuge. The increased demand for ultracentrifugation resulting from the additional faculty added to this Department over the last year (both Dr. Quinn and Dr. Pascual were hired in 1995) and in the coming year (another faculty member will be added to the Department in the next year) further emphasizes our need for the requested equipment. The Department also lacks sufficient rotors to carry out the proposed research, and this proposal includes 3 new rotors. Currently, this Department has an older fixed-angle rotor (10 years old), a swinging bucket rotor, and a derated Type 50 rotor. None of these rotors are capable of running at speeds over 60,000 r.p.m. and probably should not be used over 45,000 r.p.m. The rotors requested in this proposal are capable of much higher speeds and would facilitate faster and more efficient separations. The new equipment will be used primarily by the labs of Drs. Quinn, Jutila, Pascual, and Burgess, so this proposal contains descriptions of their projects that utilize ultracentrifugation. These projects are all funded by research grants from a number of agencies such as the N1H, NSF, and USDA. Ultracentrifugation is required as part of these projects for density gradient analysis of proteins and protein complexes, subcellular fractionation, membrane purification, and DNA purification. In addition, a small amount of centrifuge time (5%) will be available for use in Departmental classes for training students in the use of modern biological instrumentation.