Development, Characterization and Applications of Support Materials for HPLC, HPCE, CEC and SFC

这项由分析和表面化学计划资助的本科生项目的研究重点是高效液相色谱柱材料表面和开放式毛细管色谱柱壁上固定键合相的制备和表征。在这三年的持续资助中，Pesek教授和他在圣何塞州立大学的研究学生将继续研究他们为制造这些坚固的层析固定相而开发的硅烷化/硅氢化反应方案。利用漫反射红外光谱、交叉偏振魔角旋转核磁共振光谱和化学分析电子光谱等光谱方法对改性后的表面进行了表征。差示扫描量热法和热重分析也将在这项工作中使用。该项目旨在开发新的、坚固耐用的、更有效的固定相，用于分离科学。在层析分离方法中用作固定相的材料决定了层析过程是否成功，以及该过程是否可以在可接受的时间段内重现。这项本科生研究所的研究项目将包括本科生和硕士研究生参与固定相的准备，这些固定相的使用比现有材料更好。这些新的色谱固定相可用于分离有用数量的所需化合物，并对存在的化合物进行定量和鉴定。这些新型固定相在化工过程控制、合成和生物技术方面有应用。

This Research at an Undergraduate Institution project, supported in the Analytical and Surface Chemistry Program, focusses on the preparation and characterization of stationary bonded phases on high performance liquid chromatography packing material surfaces and on the walls of open capillary chromatography columns. During this three year continuing grant Professor Pesek and his research students at San Jose State University will continue studies on silanization/hydrosilation reaction schemes that they have developed for making these robust chromatography stationary phases. These modified surfaces will be characterized using the spectroscopic methods diffuse reflectance infrared spectroscopy, cross-polarization magic angle spinning nuclear magnetic resonance spectroscopy and electron spectroscopy for chemical analysis. Differential scanning calorimetry and thermal gravimetric analysis will also be used in this work. This project is intended to develop new, rugged, and more efficient stationary phases for use in separation science. The materials that serve as stationary phases in chromatographic separation methods determine whether a chromatography procedure will be successful and whether the procedure will be reproducible over an acceptable period of time. This Research at an Undergraduate Institution project will involve undergraduate and Master's level students in the preparation of stationary phases that are superior in use to existing materials. These new chromatographic stationary phases can be used to separate useful amounts of desired compounds and to quantify and identify the compounds present is a sample. These new stationary phases have application in chemical process control, synthesis, and biotechnology.