Development, Characterization and Applications of Support Materials for HPLC, HPCE, CEC and SFC

本研究是一个本科院校项目，由分析和表面化学项目支持，重点研究高效液相色谱填料表面和开放式毛细管色谱柱壁上固定键合相的制备和表征。在这三年的持续资助中，Pesek教授和他在圣何塞州立大学的研究学生将继续研究他们为制造这些强大的色谱固定相而开发的硅烷化/硅氢化反应方案。利用漫反射红外光谱、交叉极化魔角旋转核磁共振光谱和化学分析电子能谱等光谱方法对改性后的表面进行表征。差示扫描量热法和热重分析也将用于这项工作。该项目旨在开发新的，坚固的，更有效的固定相用于分离科学。在色谱分离方法中作为固定相的物质决定了色谱过程是否成功，以及该过程在可接受的时间内是否可重复。这项在本科院校进行的研究项目将涉及本科生和硕士生准备比现有材料使用更优越的固定相。这些新的色谱固定相可用于分离所需化合物的有用量，并定量和鉴定样品中存在的化合物。这些新型固定相在化学过程控制、合成和生物技术等领域有着广泛的应用。

This Research at an Undergraduate Institution project, supported in the Analytical and Surface Chemistry Program, focusses on the preparation and characterization of stationary bonded phases on high performance liquid chromatography packing material surfaces and on the walls of open capillary chromatography columns. During this three year continuing grant Professor Pesek and his research students at San Jose State University will continue studies on silanization/hydrosilation reaction schemes that they have developed for making these robust chromatography stationary phases. These modified surfaces will be characterized using the spectroscopic methods diffuse reflectance infrared spectroscopy, cross-polarization magic angle spinning nuclear magnetic resonance spectroscopy and electron spectroscopy for chemical analysis. Differential scanning calorimetry and thermal gravimetric analysis will also be used in this work. This project is intended to develop new, rugged, and more efficient stationary phases for use in separation science. The materials that serve as stationary phases in chromatographic separation methods determine whether a chromatography procedure will be successful and whether the procedure will be reproducible over an acceptable period of time. This Research at an Undergraduate Institution project will involve undergraduate and Master's level students in the preparation of stationary phases that are superior in use to existing materials. These new chromatographic stationary phases can be used to separate useful amounts of desired compounds and to quantify and identify the compounds present is a sample. These new stationary phases have application in chemical process control, synthesis, and biotechnology.