Development, Characterization and Applications of Support Materials for HPLC, HPCE, CEC and SFC

这项在本科院校项目中进行的研究得到了分析和表面化学项目的支持，重点关注高效液相色谱填充材料表面和开放毛细管色谱柱壁上固定键合相的制备和表征。 在这三年的持续资助期间，Pesek教授和他在圣何塞州立大学的研究生将继续研究硅烷化/硅氢化反应方案，他们已经开发了这些强大的色谱固定相。 这些改性的表面将使用光谱方法漫反射红外光谱，交叉偏振魔角旋转核磁共振光谱和电子光谱进行化学分析。 差示扫描量热法和热重分析也将用于这项工作。 该项目旨在开发用于分离科学的新的，坚固的，更有效的固定相。 在色谱分离方法中用作固定相的材料决定了色谱程序是否成功，以及该程序在可接受的时间内是否具有重现性。 本研究在本科院校的项目将涉及本科和硕士水平的学生在固定相的准备是上级使用现有的材料。 这些新的色谱固定相可用于分离有用量的所需化合物，并定量和鉴定样品中存在的化合物。 这些新的固定相在化学过程控制、合成和生物技术中具有应用。

This Research at an Undergraduate Institution project, supported in the Analytical and Surface Chemistry Program, focusses on the preparation and characterization of stationary bonded phases on high performance liquid chromatography packing material surfaces and on the walls of open capillary chromatography columns. During this three year continuing grant Professor Pesek and his research students at San Jose State University will continue studies on silanization/hydrosilation reaction schemes that they have developed for making these robust chromatography stationary phases. These modified surfaces will be characterized using the spectroscopic methods diffuse reflectance infrared spectroscopy, cross-polarization magic angle spinning nuclear magnetic resonance spectroscopy and electron spectroscopy for chemical analysis. Differential scanning calorimetry and thermal gravimetric analysis will also be used in this work. This project is intended to develop new, rugged, and more efficient stationary phases for use in separation science. The materials that serve as stationary phases in chromatographic separation methods determine whether a chromatography procedure will be successful and whether the procedure will be reproducible over an acceptable period of time. This Research at an Undergraduate Institution project will involve undergraduate and Master's level students in the preparation of stationary phases that are superior in use to existing materials. These new chromatographic stationary phases can be used to separate useful amounts of desired compounds and to quantify and identify the compounds present is a sample. These new stationary phases have application in chemical process control, synthesis, and biotechnology.