Silica Surface Modifications for High Performance Liquid Chromatography (HPLC) and Capillary Electrophoresis (HPCE)

这个项目是在一般地区的分析和表面 化学和分离科学的子领域。 期间 这三年的持续RUI赠款的任期，教授Pesek 他的学生们将致力于发展化学 多孔硅固定相的改性方法 高效液相色谱和玻璃相 用于高效毛细管电泳的毛细管壁。 合成方法包括将表面硅烷醇 官能化，其可以通过以下进一步反应： 硅氢化反应以产生键合的有机部分。 这些 粘结材料具有与表面的直接碳-硅键 而不是更常见的硅氧烷键， 标准有机硅烷反应程序。 的直接联系 已经在色谱和质谱上证明了 比硅氧烷键更稳定， 分析解决方案环境。 此外，结合液体 晶体固定相将进一步发展， 通过光谱、热学和 色谱上。 %%% 这项研究建立在Pesek博士的研究成果的基础上。 以前的NSF赠款，CHE-8814849，并将使一个更清晰的 移动的相/固定相界面的定义。 此外，这项研究应该提供有见地的指导， 更合理有效地设计和合成液体 结晶色谱固定相

This project is in the general area of analytical and surface chemistry and in the subfield of separation science. During the tenure of this three-year continuing RUI grant, Professor Pesek and his students will pursue the development of chemical modification procedures for alteration of porous silica stationary phases for high performance liquid chromatography and glass capillary walls for high performance capillary electrophoresis. The synthetic approach involves converting surface silanol functionalities to hydrides which can be further reacted by hydrosilation reactions to produce bonded organic moieties. These bonded materials have a direct carbon-silicon bond to the surface instead of the more common siloxane linkage which arises from standard organosilane reactions procedures. The direct linkage has been proven both chromatographically and electrophoretically to be more stable than the siloxane linkages to a much wider range of analytical solution environments. Additionally, bonded liquid crystal stationary phases will be further developed and characterized spectroscopically, thermally, and chromatographically. %%% This research builds upon results obtained under Dr. Pesek's previous NSF grant, CHE-8814849, and will enable a clearer definition of the mobile phase/stationary phase interface. Additionally, this research should provide insightful guidance for the more rational and efficient design and synthesis of liquid crystal chromatographic stationary phases.