Two-Stage Fermentation of Whey Permeate for Biodegradable Deicer Production

用于可生物降解除冰剂生产的乳清渗透物的两阶段发酵

• 批准号: 9613273
• 负责人: Alexander Mathews
• 金额: $ 24.29万
• 依托单位: Kansas State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-15 至 2000-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9613273&HistoricalAwards=false
• 关键词: Two; Stage; Fermentation; Whey; Permeate

9613273 Mathews Biomass wastes such as liquid whey effluents from the dairy industry are a burden on the environment due to their high biochemical oxygen demand (BOD). Treatment of these wastes to meet environmental standards is both capital and energy intensive. This proposal examines the use of whey permeate in the production of a road deicer substitute for sodium chloride, since currently used deicers cause extensive corrosion-related damage to the highway infrastructure and environmental damage to water supplies and soils. The proposed research will examine the use of biomass wastes in the production of substitute deicers, calcium magnesium acetate (CMA) and calcium magnesium propionate (CMP), via a two-stage bacterial fermentation process. In addition, a mathematical model will be to describe continuous cell- recycle fermentation in coculture systems. Growth kinetics studies will be conducted using the bacteria Propionibacterium acidipropionici and Lactobacillus plantarum in monoculture and coculture systems. The effect of substrate concentration, pH and other parameters on growth and organic acid productivity will be determined. A synergistic growth kinetics model that includes substrate and product inhibition kinetics will be developed, and kinetic constants will be obtained from the data. Continuous flow cell-recycle fermentation studies will be conducted for coculture and two-stage fermentation systems to determine acid productivity and product concentrations. A model will be developed to describe continuous flow cell- recycle fermentation for single and coculture systems, and the model will be verified with the experimental data. This research will develop data and basic knowledge required in the design of an innovative fermentation system for the conversion of whey effluents to useful chemicals. The methodology and approach developed can be utilized in processes to convert a variety of inexpensive sugar substrates and biomass hydrolysat es into low-cost biodegradable deicers.

小马修斯9613273 生物质废物如来自乳制品工业的液体乳清流出物由于其高生化需氧量（BOD）而对环境造成负担。处理这些废物以达到环境标准是资本和能源密集型的。 该提案审查了乳清渗透物在生产氯化钠道路除冰剂替代品中的用途，因为目前使用的除冰剂对公路基础设施造成广泛的腐蚀性损害，并对供水和土壤造成环境损害。拟议的研究将通过两阶段细菌发酵过程，研究生物质废物在生产替代除冰剂乙酸钙镁（CMA）和丙酸钙镁（CMP）中的用途。 此外，还建立了一个数学模型来描述共培养系统中的连续细胞循环发酵. 将在单一培养和共培养系统中使用细菌丙酸杆菌和植物乳杆菌进行生长动力学研究。 将确定底物浓度、pH和其他参数对生长和有机酸生产率的影响。 将开发包括底物和产物抑制动力学的协同生长动力学模型，并从数据中获得动力学常数。 将对共培养和两阶段发酵系统进行连续流细胞再循环发酵研究，以确定酸生产率和产物浓度。 将开发一个模型来描述单一和共培养系统的连续流动细胞循环发酵，并将用实验数据验证该模型。 这项研究将开发一个创新的发酵系统的设计所需的数据和基本知识，乳清废水转化为有用的化学品。 所开发的方法和途径可用于将各种廉价的糖底物和生物质水解产物转化为低成本的可生物降解的除冰剂的过程中。