Neuropeptide Analysis using Multidimensional Separation and Detection Methodology

使用多维分离和检测方法进行神经肽分析

• 批准号: 9622663
• 负责人: Alexander Scheeline
• 金额: $ 28万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9622663&HistoricalAwards=false
• 关键词: Neuropeptide; Analysis; using; Multidimensional; Separation

Urbana-Champaign This project, directed by Assistant Professor Jonathan V. Sweedler of the University of Illinois, Urbana-Champaign, is focused on identifying and quantitatively measuring neuronal releasates and assaying the contents of individual peptide vesicles. Support is provided by the Analytical and Surface Chemistry Program in the Division of Chemistry and by Neuroendocrinology in the Division of Integrative Biology and Neuroscience. The work will develop new wavelength resolved fluorescence detection methods for microseparations that allow neuropeptides and classical neurotransmitters to be identified and quantitatively measured without the need for chemical derivitization, provide methods to assay the contents of individual peptidergic vesicles, and develop a separation approach which allows the time course of peptide release to be followed. These measurements are all concerned with the dynamics of cellular communication and the studies are designed to lead to improved understanding of the nervous system. The bag cell neurons of the marine mollusk, Aplysia Californica, will serve as a model system. An understanding of the chemical pathways involved in the transmission of a nerve impulse is essential to our gaining full knowledge of how the nervous system functions. This work will explore such pathways by developing the measurement techniques needed to follow peptide neurotransmitters which are typically present in very low concentrations and which are found in the presence of many other biological compounds.

Urbana-Champaign该项目由助理教授Jonathan V. Sweedler指导， 伊利诺伊大学厄巴纳-香槟分校专注于识别和 定量测量神经元释放物并测定 单个肽囊泡。 支持由分析和 化学系表面化学课程 综合生物学与神经内分泌学 神经科学 这项工作将发展新的波长分辨荧光 用于微量分离的检测方法， 待鉴定和定量测量的经典神经递质 不需要化学衍生化，提供测定 单个肽能囊泡的内容物，并形成分离 这种方法允许跟踪肽释放的时间过程。 这些测量都与细胞的动力学有关， 沟通和研究旨在改善 了解神经系统。 海洋生物的囊细胞神经元 软体动物，Aaplasia California，将作为一个模型系统。 了解参与传播的化学途径， 神经冲动对于我们充分了解 神经系统功能。 这项工作将探索这样的途径， 开发跟踪肽的测量技术 通常以非常低的浓度存在的神经递质 并且在许多其他生物化合物的存在下发现。