SGER: Development of Levitated Drop Reactor for Chemical, Enzyme, and Nonlinear Dynamics

SGER：用于化学、酶和非线性动力学的悬浮液滴反应器的开发

• 批准号: 0852383
• 负责人: Alexander Scheeline
• 金额: $ 19.52万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0852383&HistoricalAwards=false
• 关键词: SGER; Development; Levitated; Drop; Reactor

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). The Experimental Physical Chemistry Program supports Professor Alexander Scheeline of the University of Illinois at Urbana-Champaign to develop a levitated drop reactor to study the nonlinear reaction dynamics of myeloperoxidase, an enzyme found in neutrophils and macrophages (white blood cells). Such a reactor avoids surface reactions, adsorption, and contamination which may trouble other microfluidic systems. Gas/liquid mass transfer is facile because of the large surface area-to-volume ratio of the drop. Ultrasound is used to levitate the drop, ensuring that the drop is well-mixed. Diagnostics to be optimized for use with this microreactor include molecular fluorescence, absorption, and chronoamperometry. This research gains broad impact by allowing clearer understanding of the chemical dynamics of an enzyme central to the innate immune system. Myeloperoxidase chemistry has much in common with that in chlorination of water supplies, yet that anti-bacterial, anti-viral chemistry, one of the two greatest contributors to increased longevity in human history (the other is sanitation and hand washing by health care professionals), is not well understood. Supported research will measure rates of reactions central to this aspect of immunochemistry. Professor Scheeline's group addresses the national infrastructure needs for a diverse scientifically-literate work force through outreach activities to high school teachers, high school students, and underrepresented groups, and via active research participation by undergraduate and graduate students, women, and underrepresented groups.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。实验物理化学计划支持伊利诺伊大学厄巴纳-香槟分校的亚历山大·谢琳教授开发一种悬浮液滴反应器，以研究髓过氧化物酶的非线性反应动力学，髓过氧化物酶是一种在中性粒细胞和巨噬细胞（白色血细胞）中发现的酶。 这样的反应器避免了可能困扰其他微流体系统的表面反应、吸附和污染。 气/液传质是容易的，因为大的表面积与体积比的液滴。 超声波用于悬浮液滴，确保液滴充分混合。 与此微反应器使用的诊断优化包括分子荧光，吸收，计时电流法。这项研究通过更清楚地了解先天免疫系统核心酶的化学动力学而产生了广泛的影响。 髓过氧化物酶化学与氯化供水有很多共同之处，但抗菌，抗病毒化学，人类历史上延长寿命的两个最大贡献者之一（另一个是卫生保健专业人员的卫生和洗手），还没有得到很好的理解。 所支持的研究将测量对免疫化学这一方面至关重要的反应速率。 Scheeline教授的小组通过对高中教师，高中学生和代表性不足的群体的外展活动，并通过本科生和研究生，妇女和代表性不足的群体积极参与研究，解决了国家基础设施对多样化科学素养劳动力的需求。