CAREER: A Biomimetic Active Separation Device Based on the Microtubule Motor Protein, Kinesin

职业：基于微管运动蛋白驱动蛋白的仿生主动分离装置

• 批准号: 9624907
• 负责人: Russell Stewart
• 金额: $ 24.57万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-15 至 2001-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9624907&HistoricalAwards=false
• 关键词: CAREER; Biomimetic; Active; Separation; Device

CTS - 9624907 Russell Stewart University of Utah ABSTRACT The project is on the fabrication of a microanalytical separation device incorporating the microtubular motor protein (kinesin) into nano-fabricated machines. A prototype active chromatography device will be built to recognize, separate and detect specific DNA fragments on a single micromachined chip. A fluorescent-labeled DNA fragment in solution will attach selectively to the motor protein and move along aligned microtubules in a microchannel to a detector. The potential impact of the project is strong in bioseparations and chemical process monitoring. If successful, it may be possible to mimic intracellular transport in fabricated machines. The educational plan includes development of a new lecture and two laboratory courses on protein engineering and microfabrication. Students will be trained to apply biological principles to conventional engineering problems.

CTS-9624907犹他州拉塞尔·斯图尔特大学摘要该项目是关于将微管运动蛋白(Kinesin)融入纳米制造机器中的微分析分离装置的制造。将建立一个原型活性层析设备，以识别、分离和检测单个微机械芯片上的特定DNA片段。溶液中的荧光标记DNA片段将选择性地附着在马达蛋白上，并沿着微通道中排列的微管移动到检测器。该项目在生物分离和化学过程监测方面的潜在影响很大。如果成功，就有可能在制造的机器中模拟细胞内的运输。该教育计划包括开发一门新的讲座和两门关于蛋白质工程和微制造的实验室课程。学生将接受将生物学原理应用于常规工程问题的培训。