Controlled Fluorescent Derivatization of Peptides and Proteins for Characterization by Capillary Electrophoresis Techniques

这项由分析和表面化学计划支持的教师早期职业发展(CALEAR)项目专注于开发新的、有用的荧光标记策略，使生物大分子分析成为可能，特别是使用毛细管电泳法(CE)进行蛋白质和多肽的分析。在这项为期四年的持续资助期间，Beale教授和他在伯明翰阿拉巴马大学的学生将开发一种使用CE和激光诱导荧光(LIF)检测的高灵敏度肽图绘制方法，研究部分特定的多肽切割方案，并寻求在不修改整个蛋白质的情况下在蛋白质的N端放置单一荧光标记的策略。在这一资助期间，比尔教授将让本科生获得研究机会，并在高级分析课程中实施基于项目的实验室经验。这门课程将为法医学、公共卫生、医学预科和化学专业的学生量身定做。通过绘制多肽序列来表征蛋白质的能力和开发灵敏的蛋白质分离方法是生物技术中许多应用的重要目标。将要开发的基于CE和LIF的分离将是这一职业研究项目的敏感和实际成果。本科生分析课程的丰富和本科生参与前沿研究项目的更多机会是这一职业研究项目的教育方面，将对阿拉巴马大学伯明翰分校的学生产生积极影响。

This Faculty Early Career Development (CAREER) project, supported in the Analytical and Surface Chemistry Program, focusses on the development of novel, useful, fluorescent labeling strategies that will permit biomacromolecule analysis, especially for proteins and peptides, using capillary electrophoresis (CE). During the tenure of this four-year continuing grant, Professor Beale and his students at the University of Alabama at Birmingham will develop a highly sensitive peptide mapping protocol using CE with laser-induced fluorescence (LIF) detection, investigate partial specific peptide cleavage schemes and pursue strategies for putting a single fluorescent label at the n-terminus of a protein without modifying the entire protein. During this grant period Professor Beale will expose undergraduates to research opportunities and implement project-based laboratory experiences in the senior level analytical course. This course will be tailored to students in forensic science, public health, pre-med and chemistry. The ability to characterize proteins by mapping peptide sequences and to develop sensitive protein separation methods are important objectives for a host of applications in biotechnology. The separations based on CE with LIF to be developed will be sensitive and practical outcomes of this CAREER research project. The enrichment of the undergraduate analytical curriculum and the enhanced opportunities for undergraduates to participate in frontier research projects are educational aspects of this CAREER research project that will positively impact students at the University of Alabama at Birmingham.