Controlled Fluorescent Derivatization of Peptides and Proteins for Characterization by Capillary Electrophoresis Techniques

肽和蛋白质的受控荧光衍生化，用于通过毛细管电泳技术进行表征

• 批准号: 9624884
• 负责人: Larry Krannich
• 金额: $ 24.65万
• 依托单位: University of Alabama at Birmingham
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9624884&HistoricalAwards=false
• 关键词: Controlled; Fluorescent; Derivatization; Peptides; Proteins

This Faculty Early Career Development (CAREER) project, supported in the Analytical and Surface Chemistry Program, focusses on the development of novel, useful, fluorescent labeling strategies that will permit biomacromolecule analysis, especially for proteins and peptides, using capillary electrophoresis (CE). During the tenure of this four-year continuing grant, Professor Beale and his students at the University of Alabama at Birmingham will develop a highly sensitive peptide mapping protocol using CE with laser-induced fluorescence (LIF) detection, investigate partial specific peptide cleavage schemes and pursue strategies for putting a single fluorescent label at the n-terminus of a protein without modifying the entire protein. During this grant period Professor Beale will expose undergraduates to research opportunities and implement project-based laboratory experiences in the senior level analytical course. This course will be tailored to students in forensic science, public health, pre-med and chemistry. The ability to characterize proteins by mapping peptide sequences and to develop sensitive protein separation methods are important objectives for a host of applications in biotechnology. The separations based on CE with LIF to be developed will be sensitive and practical outcomes of this CAREER research project. The enrichment of the undergraduate analytical curriculum and the enhanced opportunities for undergraduates to participate in frontier research projects are educational aspects of this CAREER research project that will positively impact students at the University of Alabama at Birmingham.

该学院早期职业发展（CAREER）项目，在分析和表面化学计划的支持下，专注于开发新的，有用的，荧光标记策略，这将允许生物大分子分析，特别是蛋白质和肽，使用毛细管电泳（CE）。 在为期四年的持续资助的任期内，比尔教授和他的学生在伯明翰的亚拉巴马大学将开发一个高灵敏度的肽映射协议，使用CE与激光诱导荧光（LIF）检测，研究部分特定的肽切割方案，并追求战略，把一个单一的荧光标记在N-末端的蛋白质，而不修改整个蛋白质。 在此期间，比尔教授将让本科生接触研究机会，并在高级分析课程中实施基于项目的实验室经验。 本课程将为法医学，公共卫生，医学预科和化学专业的学生量身定制。 通过绘制肽序列来表征蛋白质的能力和开发灵敏的蛋白质分离方法的能力是生物技术中许多应用的重要目标。 基于CE与LIF的分离将是该CAREER研究项目的敏感和实用成果。 本科分析课程的丰富和增强本科生参与前沿研究项目的机会是这个职业研究项目的教育方面，这将对伯明翰亚拉巴马大学的学生产生积极影响。