Adaptations of Cyanobacteria to High Solar Irradiance: UV Tolerance "Strategies"

蓝藻对高太阳辐照度的适应：紫外线耐受“策略”

• 批准号: 9630674
• 负责人: Richard Castenholz
• 金额: $ 31.18万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-15 至 1999-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9630674&HistoricalAwards=false
• 关键词: Adaptations; Cyanobacteria; Solar; Irradiance; UV

9630674 Castenholz The goals of this project are to evaluate the negative effects of ultra-violet (UV) radiation on photosynthesis, growth, and health of cyanobacteria and to determine the methods used by these microorganisms to survive UV stress. Cyanobacteria (also called blue-green algae) are the closest living relatives of those oxygen-producing microbes that built the Precambrian stromatolites (1 -3 billion yrs ago) and that accounted eventually for the high oxygen values in the Earth's atmosphere. The investigators are asking the question of what biological "strategies" these microorganisms use to tolerate current UV stress levels and the higher levels which would have been prevalent during this strictly microbial period of the Earth's history. They have been working on avoidance responses by motile cells and also on the effect of UV "sunscreen" pigments, one of which (scytonemin), is located in a sheath or capsule surrounding the cells of many species. The investigators have been able to characterize this yellow pigment and experimentally show its great effectiveness in allowing cells to tolerate high UV levels. This pigment enhances survival of cyanobacteria under the high solar radiation of their natural habitats and may have provided the protection for their growth, colonization, and evolution during the Precambrian. By leaming how specific organisms protect themselves from this harmful short-wave radiation, we may also better understand the nature of UV damage in other organisms. Basic research of the type we are doing may lead to applications in human health as well. For example, as a result of previous work by the principal invesigator, scytonemin is now undergoing clinical tests in another laboratory as a compound with highly effective anti-inflammatory properties. In the continuation of this work with cyanobacteria the investig ators hope to further understand the role of scytonemin in UV protection in both actively growing and dormant cells, and the role of various environmental factors in the regulation of scytonemin synthesis. In addition, the investigators intend to determine the extent of metabolic repair processes required for UV tolerance in cyanobacteria that do not possess UV screening pigments. In this line of experimentation the investigators will also assay the degree of damage to cells caused by "reactive oxygen" (e.g. singlet oxygen) which may also be produced in cells as a result of UV absorption. If this secondary effect of UV is substantial, we will determine the role of cell carotenoid pigments (e.g. beta-carotene) in quenching this form of damage.

小行星9630674 该项目的目标是评估紫外线（UV）辐射对蓝藻光合作用，生长和健康的负面影响，并确定这些微生物在UV胁迫下生存的方法。 蓝细菌（也称为蓝绿藻）是那些产氧细菌的近亲。 微生物 的 建造 的 前寒武纪叠层石 （1 - 3亿年前）， 最终解释了地球大气中的高含氧量。 研究人员提出的问题是，这些微生物使用什么生物“策略”来耐受当前的紫外线应力水平以及在地球历史上这个严格的微生物时期普遍存在的更高水平。 他们一直在研究运动细胞的回避反应，以及紫外线“防晒”色素的作用，其中之一（scytonemin）位于许多物种细胞周围的鞘或胶囊中。研究人员已经能够描述这种黄色色素的特征，并通过实验证明其在允许细胞耐受高紫外线水平方面的巨大有效性。 这种色素增强了蓝藻在其自然栖息地的高太阳辐射下的生存能力，并可能为它们在前寒武纪的生长，殖民和进化提供了保护。 通过了解特定的有机体如何保护自己免受这种有害的短波辐射，我们也可以 更好地了解其他生物体中紫外线损伤的性质。 我们正在进行的这类基础研究也可能导致在人类健康方面的应用。 例如，由于主要研究者以前的工作，scytonemin现在正在另一个实验室进行临床试验，作为一种具有高效抗炎特性的化合物。 在 这项研究的继续是蓝藻， 研究人员希望进一步了解在活跃生长和休眠细胞中，scytonemin在紫外线防护中的作用，以及各种环境因素在调节scytonemin合成中的作用。 此外，研究人员打算确定不具有UV屏蔽色素的蓝藻中UV耐受性所需的代谢修复过程的程度。 在这一系列实验中，研究人员还将测定由“活性氧”（例如单线态氧）引起的细胞损伤程度，活性氧也可能因紫外线吸收而在细胞中产生。 如果紫外线的这种次级效应是实质性的，我们将确定细胞类胡萝卜素色素（例如β-胡萝卜素）在淬灭这种形式的损伤中的作用。