Ethylene-forming Enzyme: Structure and Mechanism

乙烯形成酶：结构和机制

• 批准号: 9632047
• 负责人: Michael Pirrung
• 金额: $ 37.2万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-15 至 2000-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9632047&HistoricalAwards=false
• 关键词: Ethylene; forming; Enzyme; Structure; Mechanism

The long term goal of the project is to fully understand the the mechanism of the ethylene-forming enzyme. The focus of this research is to use bioorganic methods to study the identity of critical active-site residues; to provide an unambiguous demonstration of a suspected radical mechanism; to finalize the enzymatic reaction stoichiometry; and to create a reasonable model for the mechanism of oxygen activation. Since enzymes of this class do not follow classic Pauling theories of enzymatic catalysis, the need to develop new theories of enzymatic catalysis for multi-transition state conversions provides further significance to the work. With this award the Organic Dynamics Program is supporting the research of Dr. Michael C. Pirrung of the Department of Chemistry at Duke University. Professor Pirrung will focus his work on understanding the mechanism of the ethylene-forming enzyme as an available and significant member of the class of non-heme iron oxidizing enzymes. Among other reasons, the work is deemed important since the process of natural ethylene production is a key step in the ripening of fruit.

该项目的长期目标是充分了解 乙烯形成酶的机制。 本研究的重点是 利用生物有机方法研究关键活性部位的身份 残留物;提供可疑基团的明确证明 机制;完成酶促反应化学计量;以及 为氧活化机理建立合理的模型。 以来 这类酶不遵循经典的鲍林理论， 酶催化，需要发展新的理论酶 用于多过渡态转化的催化进一步提供了 对工作的重要性。有了这个奖项，有机动力学计划是 支持Michael C.博士的研究皮龙 在杜克大学学化学。 皮龙教授将把他的工作重点放在 了解乙烯形成酶的机制， 非血红素铁氧化物类的一个重要成员 内切酶 除其他原因外，这项工作被认为是重要的，因为 天然乙烯生产过程是成熟的关键步骤， 水果.