Enhancement of a Bio-Engineering Laboratory

加强生物工程实验室

• 批准号: 9650551
• 负责人: John Wagner
• 金额: $ 1.48万
• 依托单位: TRINE UNIVERSITY, INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9650551&HistoricalAwards=false
• 关键词: Enhancement; Bio; Engineering; Laboratory

The project consists of enhancing the laboratory content of an existing biochemical engineering course. The bio-engineering laboratory is being used in three separate week-long experiments and one quarter-long independent research project. The three basic modules are as follows: (1.) Basic Techniques_safety and culture of different types of cell using sterile technique; (2.) Biomolecular Separation_protein and nucleic acid electrophoresis along with polysaccharide separation using open column GPC; (3.) Microbial Fermentation_Quantification of growth parameters and mathematically modeling of the reactor. The independent project is open ended and determined by the student within the timed budget constraints of the course. This project takes a minimum of 30 hours to complete and requires an oral presentation of the results. Major pieces of equipment required for support of this course include a water purification system, laminar flow hood, -80+C freezer, an Inverted phase contrast microscope, and a personal computer. This equipment can be utilized by other laboratory courses in biology chemistry and chemical engineering.These improvements can result in graduates of chemical engineering who are more prepared to work with scientists with bioscience backgrounds.

该项目包括加强现有生化工程课程的实验内容。生物工程实验室正在进行三个独立的为期一周的实验和一个为期四分之一的独立研究项目。三个基本模块如下：(1)。基本技术——无菌技术对不同类型细胞的安全性和培养；（2.） 生物分子分离——开柱GPC法分离蛋白、核酸电泳及多糖；(3)。微生物发酵——生长参数的量化和反应器的数学建模。独立的项目是开放式的，由学生在课程的时间预算限制内决定。这个项目至少需要30个小时才能完成，并需要口头报告结果。本课程所需的主要设备包括水净化系统，层流罩，-80+C冷冻机，倒置相差显微镜和个人电脑。该设备可用于生物化学和化学工程的其他实验课程。这些改进可以使化学工程专业的毕业生更有能力与具有生物科学背景的科学家一起工作。