Development of Bioerodible Polymer Matrices for Osteoblast Growth and Maturation

Laurencin，卡托T. BES 970786 采取跨学科的方法， 使用聚合物材料的骨再生系统， 陶瓷和细胞。 这些可能的临床应用 提出的细胞聚合物基质将在接枝领域， 萎缩性骨不连，用于外科关节融合术， 愈合 颅面缺损，并用作假体 植入物或涂层。这是我们的补助金更新计划 （7月首次融资。1990年，1993年7月更新）。 后 成功地实现了我们1990年资助赠款的目标 在这个过程中，我们开发并充分表征了骨骼培养物， 细胞 使用 细胞 文化 技术， 构造 和 特征 2维 矩阵 的 生物相容性， 可降解 聚合物，并研究细胞聚合物 互动 通过细胞附着、扩散、生长和DNA表达的现象 在实验中，我们转向三维矩阵的设计 来支持骨细胞通过我们1993年资助的赠款， 在实现我们的总体目标方面取得了显著进展。 一 新 三维 矩阵系统是 制定 和 通过 生物力学分析和电子显微镜研究，发现 与松质骨相似 骨细胞成功地 在这些基质上播种，不仅证明了它们的 能够附着和生长，但当放置在合适的 环境，证明了能够放下矿物 矩阵 最后，设计了新的方法来评估 在这些不透明的矿化过程中， 矩阵 在这项拨款建议中，这些三维的优化 通过进一步的研究， 在体外形成的矿物质基质的性质， 材料科学技术，并进一步研究性质 骨细胞对优化的三维基质的反应 使用共聚焦和免疫荧光细胞表征 我们在上一次拨款中开发的技术。 最后 将进行一系列实验以评估 这些材料单独用于骨缺损的愈合， 有骨细胞。 ***

Laurencin, Cato T. BES 970786 An interdisciplinary approach is taken for the development of systems for the regeneration of bone using polymeric materials, ceramics and cells. Possible clinical applications of these proposed cell-polymer matrices would be in areas of grafting for atrophic non-unions of bone, for surgical arthrodeses, for healing cranio-facial defects, and for use as prosthetic implants or coatings. This is a renewal proposal of our grant (first funded in July,. 1990 and renewed in July, 1993). After successfully fulfilling the objectives of our 1990 funded grant in which we developed and fully characterized cultures of bone cells using cell culture techniques, constructed and characterized 2-dimensional matrices of biocompatible, degradable polymers, and studied cell-polymer interactive phenomena via cell attachment, spread, growth and DNA expression experiments, we moved on to the design of 3-dimensional matrices for bone cell support. Through our 1993 funded grant we have made remarkable progress toward achieving our overall goals. A new 3-dmensional matrix system was formulated and by biomechanical analyses and electron microscopic studies, found to be similar to cancellous bone. Bone cells were successfully seeded onto these matrices and demonstrated not only their ability to attach and grow, but when placed under suitable environments, demonstrated the ability to lay down a mineral matrix. Finally novel methods were designed to evaluate cellular growth and phenotype in these opaque mineralizing matrices. In this grant proposal the optimization of these 3-dimensional bone bioerodible polymer systems is performed by further study of the nature of the mineral matrix formed in vitro using material science techniques, and the further study of the nature of the bone cell response to op timized 3-dimensional matrices using confocal and immunofluorescence cell characterization technology we developed during the previous grant. Finally, a series of experiments will be undertaken to evaluate the ability of these materials to effect healing of bony defects alone and with bone cells. ***