Morphogenesis in Streptomyces

Westphering 9722960本研究的主要长期目标是了解碳源分解代谢抑制、抗生素生物合成激活和链霉菌形态发育之间的调控相互联系。这个项目的一般方法是使用参与复杂碳水化合物利用的分解代谢控制基因作为工具来探索碳利用和形态发生之间的联系。利用这种方法，发现了一类新的突变株，这些突变株在分解代谢控制和形态发生方面存在缺陷，而且还过度生产抗生素。在已知的链霉菌形态突变体中，最具多效性的bldB在先前的工作过程中被证明在调节碳利用方面存在多效性缺陷。天蓝色链霉菌的分解代谢抑制、形态发生和抗生素生物合成都需要bldB基因产物，研究该基因在这些过程中的相互作用是本项目的主要重点。为了便于分离、克隆和鉴定新的突变体，转座子诱变和通用化转导方法正在发展中，以便与天蓝色链霉菌结合用于遗传分析。

Westpheling 9722960 The primary long-term objective of this study is to understand the regulatory interconnection between carbon source catabolite repression, activation of antibiotic biosynthesis and morphological development in the Streptomyces bacteria. The general approach of this project has been to use catabolite controlled genes involved in complex carbohydrate utilization as tools to probe the connection between carbon utilization and morphogenesis. Using this approach, a new class of mutants was identified that are at once defective in catabolite control and morphogenesis, and also overproduce antibiotics. The most pleiotropic of the known morphological mutants of Streptomyces, bldB, was shown during the course of previous work to be pleiotropically defective in the regulation of carbon utilization. The bldB gene product is required for catabolite repression, the initiation of morphogenesis and antibiotic biosynthesis in S. coelicolor, and the study of how this gene functions in the interconnection of these processes is a major focus of the current project. To facilitate the isolation, cloning, and characterization of new mutants, transposon mutagenesis and generalized transduction methods in S. venezuelae are being developed to be used in combination with S. coelicolor for genetic analysis.