Collaborative Research: Biomechanics of Vertebrate Hair Cells: Experimental and Computation Analyses

合作研究：脊椎动物毛细胞的生物力学：实验和计算分析

• 批准号: 9724123
• 负责人: Ellengene Peterson
• 金额: $ 34.34万
• 依托单位: Ohio University
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9724123&HistoricalAwards=false
• 关键词: Collaborative; Research; Biomechanics; Vertebrate; Hair

IBN-9724123 PI: Peterson The inner ear of vertebrates contains mechanosensory receptors called hair cells. Each cell has extending from its surface a tuft of hairlike microscopic processes called a ciliary bundle. This ciliary bundle is extremely sensitive to deflection, and the amount and direction of mechanical deflection leads to a nerve signal from the ear about head position or about sound. There are differences in bundle morphology in different parts of the ear, but a possible functional importance for these differences has remained largely speculative. This project is a collaborative bioengineering effort involving modern imaging and computational technology to clarify possible functions for this diversity. LIght and electron microscopy are used for detailed measurements of the bundles, which are incorporated into a computational finite-element model to quantify the contribution of different structural elements to bundle micromechanics. A new fiber-optic system is used for direct tests of the bundle stiffness in isolated living hair cells, to compare with model predictions for different morphological categories of hair cell bundles. Results will have impact not only on auditory and vestibular sensory biology, but more broadly on understanding echanoreception, and with a likely impact on artificial biosensors and biotechnology at the nanoscale.

IBN-9724123 PI：Peterson脊椎动物的内耳含有称为毛细胞的机械感觉受体。 每个细胞表面都有一簇毛状的微小突起，称为纤毛束。 这个睫状束对偏转极其敏感，并且机械偏转的量和方向导致来自耳朵的关于头部位置或关于声音的神经信号。 在耳朵的不同部位，束形态存在差异，但这些差异可能的功能重要性在很大程度上仍然是推测性的。 该项目是一个合作的生物工程努力，涉及现代成像和计算技术，以澄清这种多样性的可能功能。 光和电子显微镜用于详细测量的束，这是纳入一个计算有限元模型，以量化不同的结构元素束微观力学的贡献。 一种新的光纤系统用于直接测试的束刚度在孤立的活毛细胞，比较模型预测不同形态类别的毛细胞束。研究结果不仅会对听觉和前庭感觉生物学产生影响，而且会更广泛地理解机械感受，并可能对纳米级的人工生物传感器和生物技术产生影响。