MRI: Acquisition of a Confocal Microscope for Biological Research

MRI：购买用于生物学研究的共焦显微镜

• 批准号: 0115848
• 负责人: Ellengene Peterson
• 金额: $ 31.55万
• 依托单位: Ohio University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0115848&HistoricalAwards=false
• 关键词: MRI; Acquisition; Confocal; Microscope; Biological

A grant has been awarded to Dr. E. H. Peterson and four co-PIs at Ohio University for the purchase of a laser scanning confocal microscope for biological research. Confocal microscopy is an essential tool in modern life science research, particularly in molecular biology and neuroscience. With it, the investigator uses one or more lasers to illuminate tissues, and computerized imaging to record and analyze the resulting data. The confocal microscope will be configured with four laser lines and specialized software for three-dimensional image reconstruction and quantification and for measurement of physiological processes. Costs of the confocal microscope will be shared by Ohio University and the Ohio Board of Regents. The confocal microscope will serve several federally funded research programs including (a) biomechanics of receptors in the inner ear, (b) molecular analyses of plant cell wall function, (c) interactions between endothelial cells and nanoparticles that can be used for drug delivery, (d) biochemical and physiological precess in insects, (e) neural networks in simple biological systems, (f) transgenic tomato and tobacco cells, and (g) calcium dynamics in epileptic brain tissues. The new confocal microscope will serve faculty and students in three colleges and six academic departments. It provides a state-of-the-art imaging facility for graduate and undergraduate student training in a traditionally underserved area: rural Appalachia. Ohio University's primary mission is undergraduate education, and the science and engineering departments have a strong tradition of mentoring undergraduate research through intramural programs and federal grants. The new confocal microscope will be available to these students through their research projects with science and engineering faculty. In addition, it will enhance existing course offerings and research training for the university's science and engineering graduate students.

e.h. Peterson博士和俄亥俄大学的四名合作项目负责人获得了一笔资金，用于购买用于生物研究的激光扫描共聚焦显微镜。共聚焦显微镜是现代生命科学研究的重要工具，特别是在分子生物学和神经科学研究中。有了它，研究者使用一个或多个激光照射组织，并用计算机成像记录和分析结果数据。共聚焦显微镜将配备四条激光线和专门的软件，用于三维图像重建和定量以及生理过程的测量。共聚焦显微镜的费用将由俄亥俄大学和俄亥俄校董会共同承担。共聚焦显微镜将服务于几个联邦政府资助的研究项目，包括(a)内耳受体的生物力学，(b)植物细胞壁功能的分子分析，(c)内皮细胞和纳米颗粒之间的相互作用，可用于药物输送，(d)昆虫的生化和生理过程，(e)简单生物系统中的神经网络，(f)转基因番茄和烟草细胞，以及(g)癫痫脑组织中的钙动力学。新的共聚焦显微镜将服务于三个学院和六个学术部门的教职员工和学生。它为阿巴拉契亚农村地区的研究生和本科生培训提供了最先进的成像设备。俄亥俄大学的主要任务是本科教育，科学和工程部门有很强的传统，通过校内项目和联邦拨款指导本科生的研究。新的共聚焦显微镜将提供给这些学生通过他们的研究项目与科学和工程学院。此外，它将加强现有的课程设置和研究培训，为大学的科学和工程研究生。