Catalyst Discovery with Engineered Thiamin-Dependent Transketolase: Cofactor Catalysis

化学系向州立大学颁发的启动补助金 位于布法罗的纽约大学支持史蒂文·T. 潜水员 该研究的目的是通过一种新的催化剂来识别新的催化剂。 体内筛选方法。 酶系统是转酮醇酶， 焦磷酸硫胺素辅因子作为其催化机制。 通过 酵母中转酮醇酶基因的突变，开发了一种系统， 只有当催化功能恢复时， 引入新的辅因子。 设计了各种硫胺素类似物， 合成，并测试作为新的催化辅因子的活性。 的 生长表型用于发现一种新的辅因子催化剂， 体内选择策略。 该研究探索了一种新的催化剂发现的新方法， 活细胞来传达反应的价值。 工作提高了 理解结构和功能之间的关系， 酶系统

The starter grant award of the Chemistry Division to the State University of New York at Buffalo supports the research of Professor Steven T. Diver. The objective of the research is to identify new catalysts by an in vivo screening method. The enzyme system is transketolase with thiamin pyrophosphate cofactor as its catalytic machinery. Through mutation of the transketolase gene in yeast, a system is developed in which yeast grow only when catalytic function is recovered by incorporation of a new cofactor. Various thiamin analogs are designed, synthesized, and tested for activity as new catalytic cofactors. The growth phenotype is used to discover a new cofactor catalyst as an in vivo selection strategy. The research explores a new method for novel catalyst discovery, using a living cell to communicate a reaction's worthiness. The work enhances understanding of the relationship between structure and function in enzyme systems.