P-Protein Mutants: Tools to Analyze Phloem Protein Function

P-蛋白突变体：分析韧皮部蛋白功能的工具

• 批准号: 9727626
• 负责人: Gary Thompson
• 金额: $ 30.5万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-01 至 2001-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9727626&HistoricalAwards=false
• 关键词: Protein; Mutants; Tools; Analyze; Phloem

9727626 Thompson Phloem is essential for the long distance transport of photoassimilates from the sites of synthesis or remobilization to the sites of use or storage. Although phloem tissue has been well described anatomically and morphologically, many questions remain to be answered to link structural components of the translocating cells to their function. Phloem protein (P-protein) filaments form an integral network in the cytoplasm of the translocating cells in many higher plants, yet their function is unresolved. The long term goal of this project is to relate the structural features of P-proteins to phloem biology. The PIs are utilizing the divergence between P-proteins in Cucurbita and Cucumis species to determine the functional significance of conserved features within these proteins. These experiments will be the basis for a broad mutational analysis of these proteins in functional assays performed in transgenic plants. Structural components that are required for phloem filament formation will also be assessed in vitro. Experiments are being conducted to evaluate binding domains in the phloem filament protein and to determine the involvement of additional proteins, especially the role of chaperones, in filament formation. These investigations will enhance our understanding of the biological activity of P-proteins and the mechanisms by which these proteins function.

汤普生韧皮部对光同化物从合成或再动员点到使用或储存点的长距离运输至关重要。尽管韧皮部组织已经在解剖学和形态学上得到了很好的描述，但关于易位细胞的结构成分与其功能之间的联系，仍有许多问题有待解答。在许多高等植物中，韧皮部蛋白（p蛋白）丝在易位细胞的细胞质中形成一个完整的网络，但其功能尚不清楚。该项目的长期目标是将p蛋白的结构特征与韧皮部生物学联系起来。pi正在利用葫芦和黄瓜两种p蛋白之间的差异来确定这些蛋白中保守特征的功能意义。这些实验将为在转基因植物中进行的功能分析中对这些蛋白质进行广泛的突变分析奠定基础。韧皮部细丝形成所需的结构成分也将在体外进行评估。正在进行实验，以评估韧皮部丝蛋白的结合域，并确定其他蛋白质的参与，特别是伴侣蛋白在丝形成中的作用。这些研究将增强我们对p蛋白的生物活性及其作用机制的认识。