Collaborative Research: Organization of Nucleotide Diversity in the Inversions of Drosophila pseudoobscura

合作研究：果蝇反转中核苷酸多样性的组织

• 批准号: 9729144
• 负责人: Wyatt Anderson
• 金额: $ 12.38万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9729144&HistoricalAwards=false
• 关键词: Collaborative; Research; Organization; Nucleotide; Diversity

Anderson 9729144 The research goals of this project are to test hypotheses about the genetic forces that modulate variation of chromosomal alterations that modify gene order known as inversions. The chromosomal inversions on the third chromosome of Drosophila pseudoobscura have figured prominently in the literature of evolutionary biology for more than fifty years. They have proven to be a very useful model system for studies of geographic variation, natural selection, gene flow, and genetic drift. The inversions seem to represent a clear case of adaptive selection because they vary in frequency with the environment, form classical clines, and in some populations there are seasonal cycles of inversion frequency. The objective of this proposal is to determine how selection acts on the genetic contents of inversions within and among D. pseudoobscura populations. This research will discriminate between various models of selection by analyzing nucleotide diversity of nine PCR amplified markers uniformly distributed on the third chromosome of D. pseudoobscura. The nine markers will be sequenced in D. pseudoobscura strains collected from four populations that span an inversion frequency cline. Studies of molecular evolution over the past two decades have focussed on the genetic forces that shape diversity of single genes and have not considered if selection maintains assemblages of linked genes on chromosomes. It is an open question whether the association of genes on particular chromosomes within the genome happened due to selection or accident. Inversions provide an important mechanism to maintain the association of genetic diversity distributed across long distances of chromosomes. This project will provide valuable data on the genetic forces that modulate the evolution of large segments of the genome in natural populations.

Anderson 9729144 该项目的研究目标是检验有关调节染色体改变变异的遗传力的假设，这些改变改变了称为倒位的基因顺序。 五十多年来，假暗果蝇第三条染色体上的染色体倒位一直在进化生物学文献中占有重要地位。 事实证明，它们是研究地理变异、自然选择、基因流和遗传漂变的非常有用的模型系统。 这些倒转似乎代表了适应性选择的一个明显例子，因为它们的频率随环境而变化，形成了经典的顺系，并且在某些种群中存在倒转频率的季节性周期。 本提案的目的是确定选择如何作用于 D.pseudoobscura 种群内部和之间的倒位遗传内容。 本研究将通过分析均匀分布在 D.pseudoobscura 第三条染色体上的 9 个 PCR 扩增标记的核苷酸多样性来区分各种选择模型。 这九个标记将在从跨越倒转频率谱线的四个群体收集的 D.pseudoobscura 菌株中进行测序。 过去二十年对分子进化的研究主要集中在形成单个基因多样性的遗传力上，而没有考虑选择是否维持染色体上连锁基因的组合。 基因组内特定染色体上的基因关联是否是由于选择或意外而发生，这是一个悬而未决的问题。 倒位提供了维持分布在长距离染色体上的遗传多样性关联的重要机制。 该项目将提供有关调节自然群体中大部分基因组进化的遗传力的宝贵数据。