Imaging and Characterization of Single DNA Molecules Undergoing Electrophoresis. Measurements of Force and Torque on Single DNA Molecules

电泳中单个 DNA 分子的成像和表征。

• 批准号: 9118482
• 负责人: Carlos Bustamante
• 金额: $ 49.6万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-15 至 1996-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9118482&HistoricalAwards=false
• 关键词: Imaging; Characterization; Single; DNA; Molecules

The dynamics of very large DNA molecules undergoing gel electrophoresis will be studied using a fluorescence microscope developed under a previous NSF grant. The objectives of this study are: (l) to characterize DNA motion in steady and pulsed fields; (2) to determine the molecular processes involved in the trapping of these large molecules in the gel; (3) to test for the existence of "lakes" and "super-lakes" in agarose gels; and (4) to improve the computer model of DNA electrophoresis. Understanding the dynamics of megabase DNA electrophoresis may lead to improvements in this technique which is vital to gene mapping studies. The elastic and torsional properties of single DNA molecules will also be studied by attaching them at one end to paramagnetic beads and at the other end to a microscope slide. The bead will be subjected to a magnetic field. A characterization of these basic properties of DNA is necessary for an understanding of the behaviour of these molecules in their natural milieu.

DNA大分子凝胶过程中的动力学 将使用荧光显微镜研究电泳 在之前的NSF资助下开发的。 这一目标 研究包括： (l)描述DNA在稳态和脉冲场中的运动; (2)以确定捕获过程中的分子过程 凝胶中的大分子 (3)为了测试“湖泊”和“超级湖泊”的存在， 琼脂糖凝胶;和 (4)改进DNA电泳的计算机模型。 了解巨噬细胞DNA电泳的动力学可以 这对基因工程至关重要 制图研究。 单个DNA分子的弹性和扭转性质将 也可以通过将它们的一端连接到顺磁性 另一端连接到显微镜载玻片上。 珠子会 受到磁场的影响。 这些特征 DNA的基本性质对于理解 这些分子在自然环境中的行为。