Mechanical Manipulations of Single Molecules of DNA, Proteinand their Complexes
Mechanical Manipulations of Single Molecules of DNA, Proteinand their Complexes
批准号:
9631153
负责人:
Carlos Bustamante
金额:
$19.6万
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
1996
资助国家:
美国
项目状态:
已结题
起止时间:
1996-08-01 至 1998-09-02
中文摘要
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英文摘要
9631153 Bustamante This application describes a series of experiments designed to investigate the response of DNA, proteins, and their complexes when subjected to mechanical stress. These studies are a continuation of previous effects by this laboratory to employ methods such as Optical Tweezers and Scanning Force Microscopy (SFM) to investigate the mechanical response of single molecules. The proposed studies are divided into three main groups: i) Studies of the elastic response of single dsDNA molecules; ii) Studies of the elastic response of RecA-DNA filaments; and iii) Studies of the mechanical denaturation of single molecules of T4 lysozyme and titin. %%% The results of these studies will provide completely new insights into the nature and magnitude of the intramolecular forces that hold together biological structures. In fact, previously published work on the elasticity of single dsDNA molecules has already resulted in the characterization of three distinctive regimes of DNA elasticity. These three regimes are likely to play different roles in the process of recombination in vivo. The experiments proposed here will extend these studies to directly investigate the mechanical properties of the recombinant filaments and the denaturation of single protein molecules. ***
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项目类别:Continuing grant
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资助金额:$0.0万
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依托单位:
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批准号:9732140
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资助金额:$39.0万
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财政年份:1998
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负责人:Carlos Bustamante
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依托单位:
Mechanical Manipulations of Single Molecules of DNA, Proteinand their Complexes
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批准号:9896338
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项目类别:Continuing Grant
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资助金额:$8.9万
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财政年份:1998
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依托单位:
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财政年份:1994
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依托单位:
Imaging and Characterization of Single DNA Molecules Undergoing Electrophoresis. Measurements of Force and Torque on Single DNA Molecules
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项目类别:Continuing Grant
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资助金额:$49.6万
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财政年份:1992
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依托单位:
Building of a Confocal Scanning Differential Polarization Microscope
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项目类别:Continuing Grant
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资助金额:$11.71万
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依托单位:
Building of a Confocal Scanning Differential Polarization Microscope
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项目类别:Continuing Grant
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资助金额:$18.05万
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财政年份:1989
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负责人:Carlos Bustamante
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依托单位:
Higher Structure of Chromatin: Building of a Laser Scanning Polarization Microscope
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批准号:8609654
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项目类别:Continuing Grant
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资助金额:$15.38万
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财政年份:1986
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负责人:Carlos Bustamante
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依托单位:
海外基金