Building of a Confocal Scanning Differential Polarization Microscope

目前的申请是一项关于延长资助的请求， 为期两年的生物仪器补助金， 共焦差分偏振扫描 显微镜 基础光学和电子设计与建造 该仪器的研制已在我们实验室完成。 这 显微镜已经显示出它作为一种 研究复杂生物系统的分析工具 在原地。 利用这台仪器，我们首次能够 时间，以空间分辨长程手性域，其中 光收集复合体II被组织在叶绿体中 grana. 我们要求追加三年的资金 将现有文书的能力扩大到 执行： a）在特定位置的显微分光偏振测量， 对象。 目前，显微镜可以在空间上分辨 物体的光学（线性和圆形）各向异性 光的衍射极限，在一个固定的波长， 照明。 拟建仪器的建造将 使我们能够在这个区域中找到一个感兴趣的微观区域， 研究对象，并获得圆二色性和线性 局域域的二色性光谱： B）发光去极化成像。 利用的优势 共焦落射照明光学和扩展的灵敏度 极化调制和同步解调，我们提出了 开发仪器来空间分辨分子 发光标记物种的动力学。 该测量仪表 将首先应用于成像IgE的旋转动力学 肥大细胞中的受体。

The present application is a request for renewal of funding on a two-year Biological Instrumentation Grant for the construction and testing of a Confocal Differential Polarization Scanning Microscope. The basic optical and electronic design and building of this instrument has been completed in our laboratory. This microscope has already shown its enormous potential as an analytical tool in the study of complex Biological systems in-situ. Using this instrument, we have been able, for the first time, to resolve spatially the long-range chiral domains in which the Light Harvesting Complex II is organized in the chloroplast grana. We request funding for an additional period of three years to extend the capabilities of the present instrument to carry out: a) Microspectro-polarimetric measurements at a given location in the object. Presently, the microscope can spatially resolve the optical (linear and circular) anisotropy of the object to within the diffraction limits of the light, at a fixed wavelength of illumination. The building of the proposed instrumentation will allow us to locate a microscopic domain of interest in the studies object and to obtain the Circular Dichroism and linear Dichroism spectra of that localized domain: b) Luminescence Depolarization Imaging. Using the advantages of confocal epi-dillumination optics and the extended sensitivity of polarization modulation and synchronous demodulation, we propose to develop the instrumentation to spatially resolve the molecular dynamics of luminescence-labelled species. This instrumentation will be applied first to imaging the rotational dynamics of IgE receptors in mast cells.