CAREER: Kinetic and Thermodynamic Studies of Advanced Affinity Techniques for Controlling Interfacial Protein Behavior

职业：控制界面蛋白质行为的先进亲和技术的动力学和热力学研究

• 批准号: 9733310
• 负责人: Paul Van Tassel
• 金额: $ 21万
• 依托单位: Wayne State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9733310&HistoricalAwards=false
• 关键词: CAREER; Kinetic; Thermodynamic; Studies; Advanced

ABSTRACT CTS-9733310 Paul R. Van Tassel/ Wayne State U. This grant is awarded through the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigator is Dr. Paul Van Tassel of Wayne State University. An objective of the research is the elucidation of protein adsorption onto affinity surfaces, including the effects of kinetics, ligand density and ligand spacer length on protein orientation. A second objective is to develop technology to control protein placement on a surface using electric fields and specific ligand placement. The educational aspects of the PI's career development seek to integrate course development with laboratory experiments through web based interactive modules. An outreach program seeks to team up undergraduates with high school students to provide an enhanced educational experience to potential scientists and engineers. The immobilization of protein molecules at a liquid-solid interface is an important aspect of enzyme catalysis, bioseparations and biomaterials coatings. Understanding how proteins adsorb onto surfaces and developing new techniques to manipulate this adsorption process has significant implications to the biotechnology industry. ***

摘要CTS-9733310 Paul R.货车塔塞尔/韦恩州立大学。 该赠款是通过化学和运输系统司的界面，运输和分离计划授予的。首席研究员是韦恩州立大学的Paul货车Tassel博士。本研究的一个目的是阐明蛋白质吸附到亲和表面上，包括动力学，配体密度和配体间隔区长度对蛋白质取向的影响。第二个目标是开发技术，以控制蛋白质的放置在表面上使用电场和特定的配体的位置。 PI的职业发展的教育方面寻求通过基于网络的交互式模块将课程开发与实验室实验相结合。一项推广计划旨在将本科生与高中生合作，为潜在的科学家和工程师提供更好的教育体验。 蛋白质分子在液-固界面的固定化是酶催化、生物分离和生物材料涂层的重要方面。了解蛋白质如何吸附到表面上，并开发新的技术来操纵这种吸附过程对生物技术行业具有重要意义。 ***