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Structure, Function, and Expression of Tubulins, Globins, and Microtubule-Dependent Motors from Cold-Adapted Antarctic Fishes

适应寒冷的南极鱼类的微管蛋白、珠蛋白和微管依赖性运动的结构、功能和表达

基本信息

批准号：
9815381
负责人：
Harry Detrich
金额：
$ 32.47万
依托单位：
Northeastern University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1999
资助国家：
美国
起止时间：
1999-06-01 至 2002-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9815381&HistoricalAwards=false
关键词：
Structure Function Expression Tubulins Globins

项目摘要

9815381DETRICHAs the Southern Ocean cooled during the past 25 million years, the fishes of Antarctic coastal waters evolved biochemical and physiological adaptations that maintain essential cellular processes such as cytoskeletal function and gene transcription. Their microtubules, for example, assemble and function at body temperatures (-1.8 to +1 degree centigrade) well below those of temperate species. The long-range goals of this research project are to determine, at the molecular level, the adaptations that enhance the assembly of microtubules, the expression of tubulin and globin genes, and the activity of microtubule motors in this extreme thermal regime. The specific objects are three in number: 1) to determine the primary sequence changes that contribute to the efficient polymerization of Antarctic fish tubulins at low temperatures; 2) to characterize the structure, organization and promoter-driven expression of tubulin and globin genes from an Antarctic rockcod and a temperate congener; and 3) to examine the biochemical adaptation required for efficient function of the brain kinesin motor of Antarctic fishes at low temperatures. Together, these studies should reveal the molecular adaptation of Antarctic fishes that maintain efficient cytoskeletal assembly, mechanochemical motor function, and gene expression at low temperatures.

9815381DETRICH在过去的2500万年里，随着南大洋的降温，南极沿海水域的鱼类进化出了生化和生理适应，以维持细胞的基本过程，如细胞骨架功能和基因转录。例如，它们的微管在远低于温带物种的体温(-1.8至+1摄氏度)下组装和运行。该研究项目的长期目标是在分子水平上确定在这种极端高温条件下增强微管组装、微管和珠蛋白基因表达以及微管马达活动的适应性。具体的目标有三个：1)确定有助于南极鱼类微管蛋白在低温下高效聚合的初级序列变化；2)表征南极岩鳕鱼和温带同系物微管蛋白和珠蛋白基因的结构、组织和启动子驱动的表达；以及3)研究南极鱼类在低温下高效发挥脑动蛋白马达所需的生化适应。总之，这些研究应该揭示南极鱼类在低温下保持有效的细胞骨架组装、机械力化学运动功能和基因表达的分子适应。