SGER: Evolutionary Relationship of Gene Structure and Function.

SGER：基因结构和功能的进化关系。

• 批准号: 9815714
• 负责人: Georgiana May
• 金额: $ 5.45万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9815714&HistoricalAwards=false
• 关键词: SGER; Evolutionary; Relationship; Gene; Structure

The goal of this Small Grant for Exploratory research is to use genealogical approaches to identify critical amino acid substitutions during the evolution of new mating type gene function in the basidiomycete fungus Coprinus cinereus. Previously, DNA sequence and genealogical analyses were used to describe the evolutionary history of alleles at a single mating type gene, bl. The bl gene lies within the complex A mating type locus. An examination of the genes in related species and additional analyses were used to identify amino acid substitutions that may have been important for the evolution of new mating type function. Those inferences are being tested by making site-directed mutations in alleles of bl and testing for changes in mating type. If successful, thias approach will provide a new method of analyzing and obtaining functional information from sequence data sets as well as providing a greater understanding of the evolution of mating type genes.

这项用于探索性研究的小额赠款的目的是使用系谱方法来确定担子菌真菌Coprinus cinereus在新交配型基因功能进化过程中的关键氨基酸替换。此前，DNA序列和系谱分析被用来描述单个交配型基因b1的等位基因的进化历史。Bl基因位于复合体A交配型基因座内。对相关物种中的基因进行了检查，并进行了额外的分析，以确定氨基酸替换可能对新交配型功能的进化具有重要意义。这些推论正在通过对bl等位基因进行定点突变和检测交配类型的变化来检验。如果成功，Thias方法将提供一种从序列数据集中分析和获得功能信息的新方法，并提供对交配型基因进化的更好理解。