Upgrade of High-Pressure Liquid Chromatograph (HPLC) for Amino Acid Racemization (AAR) Research of Quaternary Geochronology and Paleoclimatology

9817645米勒这笔赠款为科罗拉多大学北极和阿尔卑斯研究所地质年代学研究中心的氨基酸实验室购买高压液相色谱仪(HPLC)提供部分支持。PI‘s有很长的野外和分析程序的历史，这些程序将外消旋作为解决第四纪古气候学问题的工具。新的高效液相色谱法将利用反相高效液相色谱法的最新工程和方法学进展，并将允许从地质材料中常规分离大多数氨基酸立体异构体，所需样品比以前少近两个数量级。特别是，反相高效液相色谱法具有测量D/L比值的优势，可以快速消旋天冬氨酸，为高纬度地区的环境变化提供更高的年龄和古温度分辨率。改进的数据处理和数据处理将允许更有效地利用测量。正在进行的由NSF赞助的内部和合作研究项目旨在量化加拿大北极、俄罗斯北极、挪威北极以及澳大利亚和南极洲的气候变化。对这笔赠款的支持由地球科学仪器和设施计划(EAR/IF)和大气科学的古气候计划(ATM/PC)共享。***

9817645MillerThis grant provides partial support for the costs of acquiring a High-Pressure Liquid Chromatograph (HPLC) for the Amino Acid Laboratory at the Center for Geochronologic Research, Institute of Arctic and Alpine Research, University of Colorado. The PI's have a long history of field and analytical programs that incorporate racemization as a tool to address issues in Quaternary paleoclimatology. The new HPLC will take advantage of recent engineering and methodological advances in reverse phase HPLC, and will allow routine separation of most amino acid stereoisomers from geological materials with nearly two orders of magnitude less sample than previously required. In particular, reverse phase HPLC offers the advantage of measuring D/L ratios for fast-racemizing aspartic acid, providing greater age and paleotemperature resolution for environmental change at high latitudes. Improved data processing and data handling will allow more efficient utilization of measurements. Ongoing NSF-sponsored in-house and collaborative research programs are targeted toward quantifying climate change in the Canadian Arctic, Russian Arctic, Norwegian Arctic, as well as in Australia and Antarctica. Support for this grant is shared between the Earth Sciences Instrumentation and Facilities Program (EAR/IF) and the Division of Atmospheric Sciences' Paleoclimate Program (ATM/PC). ***