SGER: Acquisition and Testing of Flow CAM for Plankton Research

9817804斯特罗姆这个探索性研究（SGER）项目的小赠款涉及新开发的流式细胞仪和显微镜（FlowCAM）的收购，并由海洋科学界进行初步测试。 FlowCAM有可能大大增强香农角海洋中心（SPMC）至少三个资助项目的正在进行的研究，从而使该中心成为FlowCAM早期“用户社区”测试的良好场所。FlowCAM的设计目的是在相对稀的悬浮液中检查浮游藻类和原生动物种群。 它结合了散射和荧光信息与视频分析的细胞通过成像路径，通过使用一种新的透镜，允许自动成像的单细胞在一个广泛的焦平面。它允许快速和准确的细胞群计数，它获得的大小光谱，并检查在放牧实验中的荧光特性在现实中低细胞浓度。

9817804StromThis Small Grants for Exploratory Research (SGER) project involves the acquisition of the newly developed Flow Cytometer and Microscope (FlowCAM), and initial testing by the ocean science community. The FlowCAM has the potential to greatly enhance on-going research in at least three funded projects at the Shannon Point Marine Center (SPMC), thus making the Center a good site for the early "user community" testing of the FlowCAM. FlowCAM has been designed to examine planktonic algal and protozoan populations in relatively dilute suspensions. It combines scattering and fluorescence information with video analysis of cells passing the imaging path by using a novel lens which allows automated imaging of single cells in a broad focal plane. It allows quick and accurate counts of cell populations, it obtains size spectra, and examines fluorescent properties during grazing experiments at realistically low cell concentrations.