FSML: A culture facility for small marine organisms at Shannon Point Marine Center

FSML：香农角海洋中心的小型海洋生物养殖设施

• 批准号: 2014617
• 负责人: Suzanne Strom
• 金额: $ 18.24万
• 依托单位: Western Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2021-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2014617&HistoricalAwards=false
• 关键词: FSML; culture; facility; small; marine

The Shannon Point Marine Center (SPMC; https://www.wwu.edu/spmc/) has historically relied on culture methods to study how small marine organisms grow, feed, and respond to environmental pressures such as ocean acidification. Single-celled algae and non-photosynthetic protists are also used as foods for other marine organisms that are the focus of research at SPMC, including larger zooplankton, endangered pinto abalone, and threatened Pacific herring. This award supports the acquisition of equipment used for culturing small marine organisms, particularly single-celled planktonic (free-floating) algae and their direct consumers. The new culture facility will be used by students at a variety of levels in both classroom and independent research settings, and will involve new faculty from the main campus in research at the marine laboratory. The facility will also support increased collaboration with area resource management. The new instrumentation will enable isolation and identification of small marine organisms (stereomicroscope), maintenance of these organisms in culture (environmental chambers, laminar flow hood, autoclave) and characterization of their abundance and condition (flow cytometer). Research areas at SPMC that are dependent on the ability to isolate and culture small marine organisms include protist chemical signaling and defense ecology, ocean acidification effects on regional zooplankton and larval fish biology, invertebrate developmental biology and ecology, chemical defense studies, and symbiosis research. New research areas that will require the requested isolation and culturing facilities include culture of endangered pinto abalone and expansion of ocean acidification studies to threatened stocks of Pacific herring. Student involvement with the culture facility will occur through multiple avenues, including a summer REU program (pending), our graduate Marine and Estuarine Science program, and the capstone projects envisioned for the new undergraduate major in Marine and Coastal Sciences. Several of the projects that will use the culture facility have regional conservation impacts, including testing larval herring diet-development relationships, and developing methods for culture of endangered pinto abalone. Through these and other avenues, the culture facility will form or strengthen existing partnerships with area agencies and stakeholders who have interest in aquaculture pilot projects (e.g. Puget Sound Restoration Fund, Washington Department of Ecology) or environmental impact research (e.g. WA Ocean Acidification Center). Finally, the culture facility will improve our ability to provide cultures of heterotrophic dinoflagellates and ciliates to researchers worldwide, a service that has led to numerous significant publications in plankton ecology, biogeochemistry, and phylogenetics.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

香农角海洋中心（SPMC; https://www.wwu.edu/spmc/）历史上依靠培养方法来研究小型海洋生物如何生长，喂养，喂养和应对海洋酸化等环境压力。单细胞藻类和非光合合成生物也被用作其他海洋生物的食物，这些食物是SPMC研究的重点，包括较大的浮游动物，濒临灭绝的Pinto鲍鱼和威胁太平洋鲱鱼。该奖项支持收购用于培养小型海洋生物的设备，尤其是单细胞浮游（自由浮动）藻类及其直接消费者。新的文化设施将在课堂和独立研究环境中的各个层面上使用新的文化设施，并将涉及主要校园的新教师在海洋实验室的研究中。该设施还将支持与地区资源管理的增加。新的仪器将能够隔离和鉴定小型海洋生物（立体显微镜），在培养物中维持这些生物（环境腔，层流，层流罩，高压灭菌）以及其丰度和状况（流式细胞仪）的表征。 SPMC的研究领域取决于分离和培养小型海洋生物的能力，包括原生物学信号传导和国防生态学，海洋酸化对区域浮游生物和幼虫鱼类生物学的影响，无脊椎动物发育生物学和生态学，化学辩护研究，化学辩护研究和共生研究研究。需要要求隔离和培养设施的新研究领域包括濒临灭绝的pinto鲍鱼的培养以及将海洋酸化研究扩展到威胁太平洋鲱鱼的股票。学生参与文化设施将通过多种途径进行，包括夏季REU计划（待定），我们的毕业生海洋和河口科学计划，以及为海洋和沿海科学领域的新本科专业设想的Capstone Projects。使用文化设施的一些项目具有区域保护的影响，包括测试幼虫鲱鱼饮食发展关系以及开发濒危Pinto鲍鱼培养的方法。通过这些和其他途径，文化设施将与对水产养殖试点项目感兴趣的地区机构和利益相关者建立或加强现有的合作伙伴关系（例如华盛顿生态学系普吉特海湾恢复基金）或环境影响研究（例如，WA Oceal Acidification Center）。最后，文化机构将提高我们提供异育鞭毛素和纤毛文化的能力，向全球研究人员提供纤毛，这项服务导致了浮游生物生态学，生物地球化学和系统发育学上的许多重要出版物，该奖项反映了NSF的法定任务，并通过评估范围来反映出构成群体的支持者，该奖项被视为众所周知的范围。