The Use of the Isothermal Titration Microcalorimeter to Enhance the Undergraduate Chemistry and Biochemistry Laboratory

购买最先进的等温滴定微量热计(ITC)将使学生有机会在一次实验中通过实验确定所研究系统的完整热力学轮廓，从而极大地加强多门课程的教学。具体地说，ITC将提供以下能力：在物理化学和生物化学课程中以更有意义的方式教授溶液热力学；在一些课程的实验室计划中进行激动人心的相关实验；以及开发需要使用溶液微量热计的本科研究项目。从物理化学到生物化学的各种课程都建议进行一些新的实验。这些包括：核糖核酸酶和核糖核酸酶衍生物与底物类似物CMP的相互作用；碳水化合物与凝集素的结合；金属离子与四环素的相互作用；β-半乳糖苷酶和KI的Km的测定；一些-半乳糖苷酶抑制剂的Km的测定；氰化物和叠氮化物与高铁肌红蛋白的结合。国际贸易中心的使用将使本科生接触到这些重要的化学和生物化学领域，这是目前的设备不可能有效做到的。

The acquisition of a state-of-the-art isothermal titration microcalorimeter (ITC) will greatly enhance instruction in a number of classes by providing the students with the opportunity to experimentally determine a complete thermodynamic profile for the system under investigation in one experiment. Specifically, the ITC will provide the capability to: teach solution thermodynamics in the physical chemistry and biochemistry courses in a more meaningful way; perform exciting and relevant experiments in the laboratory programs of a number of courses; and develop undergraduate research projects which require the use of an solution microcalorimeter. A number of new experiments are proposed for courses ranging from physical chemistry to biochemistry. These include: the interaction of ribonuclease and ribonuclease derivatives with the substrate analogue CMP; the binding of carbohydrates to lectins; the interaction of metal ions with tetracycline; the determination of the Km for ?-galactosidase and the KI a number of inhibitors of (-galactosidase; and the binding of cyanide and azide to metmyoglobin. The use of the ITC will expose undergraduate students to these important areas of chemistry and biochemistry which is not possible to do effectively with the present equipment.