The Use of the Isothermal Titration Microcalorimeter to Enhance the Undergraduate Chemistry and Biochemistry Laboratory

获得最先进的等温滴定微热计（ITC）将极大地加强许多课程的教学，为学生提供在一个实验中实验确定所研究系统的完整热力学剖面的机会。具体而言，ITC将提供以下能力：以更有意义的方式教授物理化学和生物化学课程中的溶液热力学；在一些课程的实验室项目中进行令人兴奋的相关实验；开发需要使用溶液微量热计的本科生研究项目。从物理化学到生物化学等课程都提出了许多新的实验。这些包括：核糖核酸酶和核糖核酸酶衍生物与底物类似物CMP的相互作用；凝集：碳水化合物与凝集素的结合；金属离子与四环素的相互作用；Km的确定？-半乳糖苷酶和KI有一些-半乳糖苷酶的抑制剂，以及氰化物和叠氮化物与肌红蛋白的结合。ITC的使用将使本科生接触到化学和生物化学的这些重要领域，而这些领域是不可能用现有设备有效地完成的。

The acquisition of a state-of-the-art isothermal titration microcalorimeter (ITC) will greatly enhance instruction in a number of classes by providing the students with the opportunity to experimentally determine a complete thermodynamic profile for the system under investigation in one experiment. Specifically, the ITC will provide the capability to: teach solution thermodynamics in the physical chemistry and biochemistry courses in a more meaningful way; perform exciting and relevant experiments in the laboratory programs of a number of courses; and develop undergraduate research projects which require the use of an solution microcalorimeter. A number of new experiments are proposed for courses ranging from physical chemistry to biochemistry. These include: the interaction of ribonuclease and ribonuclease derivatives with the substrate analogue CMP; the binding of carbohydrates to lectins; the interaction of metal ions with tetracycline; the determination of the Km for ?-galactosidase and the KI a number of inhibitors of (-galactosidase; and the binding of cyanide and azide to metmyoglobin. The use of the ITC will expose undergraduate students to these important areas of chemistry and biochemistry which is not possible to do effectively with the present equipment.