The Use of the Isothermal Titration Microcalorimeter to Enhance the Undergraduate Chemistry and Biochemistry Laboratory

使用等温滴定微量热仪增强本科生化学与生物化学实验室

• 批准号: 9850820
• 负责人: Lawrence Kaplan
• 金额: $ 3.48万
• 依托单位: Williams College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9850820&HistoricalAwards=false
• 关键词: Use; Isothermal; Titration; Microcalorimeter; Enhance

The acquisition of a state-of-the-art isothermal titration microcalorimeter (ITC) will greatly enhance instruction in a number of classes by providing the students with the opportunity to experimentally determine a complete thermodynamic profile for the system under investigation in one experiment. Specifically, the ITC will provide the capability to: teach solution thermodynamics in the physical chemistry and biochemistry courses in a more meaningful way; perform exciting and relevant experiments in the laboratory programs of a number of courses; and develop undergraduate research projects which require the use of an solution microcalorimeter. A number of new experiments are proposed for courses ranging from physical chemistry to biochemistry. These include: the interaction of ribonuclease and ribonuclease derivatives with the substrate analogue CMP; the binding of carbohydrates to lectins; the interaction of metal ions with tetracycline; the determination of the Km for ?-galactosidase and the KI a number of inhibitors of (-galactosidase; and the binding of cyanide and azide to metmyoglobin. The use of the ITC will expose undergraduate students to these important areas of chemistry and biochemistry which is not possible to do effectively with the present equipment.

一个国家的最先进的等温滴定微量热计（ITC）的收购将大大提高教学在一些类通过提供学生有机会在实验中确定一个完整的热力学配置文件的系统正在调查中的一个实验。具体来说，ITC将提供以下能力：以更有意义的方式教授物理化学和生物化学课程中的溶液热力学;在多门课程的实验室项目中进行令人兴奋且相关的实验;并开发需要使用的本科生研究项目溶液微量热计。为从物理化学到生物化学的课程提出了一些新的实验。其中包括：核糖核酸酶和核糖核酸酶衍生物与底物类似物CMP的相互作用;碳水化合物与凝集素的结合;金属离子与四环素的相互作用;？-半乳糖苷酶和KI是β-半乳糖苷酶的许多抑制剂;氰化物和叠氮化物与高铁肌红蛋白的结合。ITC的使用将使本科生接触到化学和生物化学的这些重要领域，而这些领域是目前设备无法有效完成的。