MRI:Acquisition of an Isothermal Titration Calorimeter to Characterize Molecular Interactions

巴克内尔大学的David Rovnyak教授和他的同事Thomas Selby、Marie Pizzorno、Timothy Strein和Charles Clapp获得了化学专业研究仪器计划颁发的这一奖项，他们将获得一台等温滴定量热仪(ITC)。该建议旨在加强所有层面的研究和教育，特别是在以下领域：(A)用微量热法研究分子相互作用；(B)胆汁胶束与客体的结合；(C)病毒蛋白之间蛋白质-蛋白质相互作用的研究；(D)大豆脂肪氧合酶-1抑制剂的表征；以及(E)磷脂酶C(PI-PLC)酶的结合和专一性。等温滴定量热法(ITC)是一种非常敏感的热力学技术，用于表征物种之间的结合作用，如小分子和酶之间的结合作用。ITC直接测量在装订过程中吸收或释放的热量。这提供了对化学和生化结合相互作用强度的洞察。该量热计还将被并入几个高级师级别的实验室班级。因此，这一工具不仅将用于研究，而且还将用于教育和外联活动。

With this award from the Chemistry Major Research Instrumentation Program, Professor David Rovnyak from Bucknell University and colleagues Thomas Selby, Marie Pizzorno, Timothy Strein and Charles Clapp will acquire an isothermal titration calorimeter (ITC). The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) study of molecular interactions by microscale calorimetry; (b) guest-host binding by bile micelles; (c) study of protein-protein interactions between viral proteins; (d) characterization of inhibitors of soybean lipoxygenase-1; and (e) binding and specificity of phosphoinositide-specific phospholipase C (PI-PLC) enzymes.Isothermal titration calorimetry (ITC) is a very sensitive thermodynamic technique that is used to characterize binding interactions between species such as those between a small molecule and an enzyme. ITC measures directly the heat absorbed or released during a binding process. This provides insight on the strength of chemical and biochemical binding interactions. The calorimeter will also be incorporated into several upper division level laboratory classes. Thus this instrument will be used not only in research but also in educational and outreach activities.