MRI:Acquisition of an Isothermal Titration Calorimeter to Characterize Molecular Interactions

MRI：获取等温滴定量热计来表征分子相互作用

• 批准号: 1337721
• 负责人: David Rovnyak
• 金额: $ 9.1万
• 依托单位: Bucknell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337721&HistoricalAwards=false
• 关键词: MRI; Acquisition; Isothermal; Titration; Calorimeter

With this award from the Chemistry Major Research Instrumentation Program, Professor David Rovnyak from Bucknell University and colleagues Thomas Selby, Marie Pizzorno, Timothy Strein and Charles Clapp will acquire an isothermal titration calorimeter (ITC). The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) study of molecular interactions by microscale calorimetry; (b) guest-host binding by bile micelles; (c) study of protein-protein interactions between viral proteins; (d) characterization of inhibitors of soybean lipoxygenase-1; and (e) binding and specificity of phosphoinositide-specific phospholipase C (PI-PLC) enzymes.Isothermal titration calorimetry (ITC) is a very sensitive thermodynamic technique that is used to characterize binding interactions between species such as those between a small molecule and an enzyme. ITC measures directly the heat absorbed or released during a binding process. This provides insight on the strength of chemical and biochemical binding interactions. The calorimeter will also be incorporated into several upper division level laboratory classes. Thus this instrument will be used not only in research but also in educational and outreach activities.

有了这个奖项从化学主要研究仪器计划，教授大卫Rovnyak从巴克内尔大学和同事托马斯塞尔比，玛丽Pizzorno，蒂莫西Strein和查尔斯克拉普将获得等温滴定量热仪（ITC）。该提案旨在加强各级的研究和教育，特别是在以下领域：（a）用微量量热法研究分子间的相互作用;（B）胆汁胶束的宾主结合;（c）研究病毒蛋白质之间的蛋白质间相互作用;（d）大豆脂氧合酶-1抑制剂的特性;（e）大豆脂氧合酶-1抑制剂的特性。和（e）磷酸肌醇特异性磷脂酶C（PI-PLC）酶的结合和特异性。是一种非常敏感的热力学技术，用于表征物质之间的结合相互作用，例如小分子和酶之间的结合相互作用。ITC直接测量装订过程中吸收或释放的热量。这提供了对化学和生物化学结合相互作用的强度的洞察。热量计也将被纳入几个较高的师级实验室类。因此，该仪器不仅将用于研究，还将用于教育和外展活动。