MRI: Acquisition of an Isothermal Titration Calorimeter for the Characterization of Molecular Interactions to Support Research and Undergraduate Education

MRI：购买等温滴定量热仪用于表征分子相互作用以支持研究和本科教育

• 批准号: 1531247
• 负责人: Veronica Moorman
• 金额: $ 9.16万
• 依托单位: Kettering University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531247&HistoricalAwards=false
• 关键词: MRI; Acquisition; Isothermal; Titration; Calorimeter

With this award from the Major Research Instrumentation (MRI) Program (MRI) and the Chemistry Research Instrumentation and Facilities (CRIF) Program, Kettering University will acquire an isothermal scanning calorimeter (ITC). Isothermal titration calorimetry has become a powerful technique for obtaining quantitative thermodynamic information about molecular interactions. It does so by sensitively measuring heat generated or absorbed by a compound upon titration using another compound. This leads to understanding the strength and structure of the interaction between the compounds. Binding between proteins, nucleic acids, lipids, small molecules, metals, nanoparticles, and polymers dissolved or suspended in aqueous or select non-aqueous solvents are accessible, making ITC extremely versatile. At Kettering University, the instrument will also be used by undergraduate students in their research projects. It will be incorporated into laboratory courses to provide training to students in several disciplines. It will be used in demonstrations and in hands on usage in outreach programs for middle and high school students. The proposal is aimed at enhancing research especially in areas such as (a) investigating protein-small molecule binding to explain the molecular evolution of the Yersina phosphatase YopH; (b) elucidating potential cytochrome c-dependent cardiovascular disease protection mechanisms for catechins, protein-metal complex binding; (c) determining the affinity of blood serum proteins for anti-cancer Ru(II)-mono-arene complexes; (d) determining protein-RNA binding for RNA sequence specificity of a T. brucei protein known to regulate gene expression and (e) studying small molecule-nanoparticle binding to understand the thermal properties of sodium acetate trihydrates for their use in non-electric incubating blankets.

凭借主要研究仪器（MRI）计划（MRI）和化学研究仪器和设施（CRIF）计划的这一奖项，凯特林大学将获得等温扫描量热仪（ITC）。等温滴定量热法已成为获得分子间相互作用的定量热力学信息的有力手段。它通过灵敏地测量在使用另一种化合物滴定时由一种化合物产生或吸收的热量来实现。这有助于了解化合物之间相互作用的强度和结构。溶解或悬浮在水性或选择的非水性溶剂中的蛋白质、核酸、脂质、小分子、金属、纳米颗粒和聚合物之间的结合是可获得的，使得ITC极其通用。在凯特林大学，该仪器也将用于本科生在他们的研究项目。它将被纳入实验室课程，为学生提供几个学科的培训。它将用于演示和在初中和高中学生的推广计划中的实际使用。该提案旨在加强研究，特别是在以下领域的研究：（a）研究蛋白质-小分子结合，以解释耶尔森氏菌磷酸酶YopH的分子进化;（B）阐明儿茶素、蛋白质-金属络合物结合的潜在细胞色素c依赖性心血管疾病保护机制;（c）确定血清蛋白质对抗癌Ru（II）-单芳烃络合物的亲和力;（d）确定血清蛋白质对抗癌Ru（II）-单芳烃络合物的亲和力。（d）确定T.已知调节基因表达的布氏杆菌蛋白，以及（e）研究小分子-纳米颗粒结合以了解三水合乙酸钠的热性质，以用于非电孵育毯。