Efficacy of virus detection and elimination in natural waters and sediments - laboratory and technical scale investigations

天然水体和沉积物中病毒检测和消除的功效 - 实验室和技术规模调查

• 批准号: 130579553
• 负责人: Privatdozent Dr. Hans-Christoph Selinka
• 金额: --
• 依托单位: Abteilung II 1: Umwelthygiene
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/130579553?language=en
• 关键词: Efficacy; virus; detection; elimination; natural

Public health protection against human pathogenic viruses in water needs more efficient virus enrichment techniques and sensitive detection methods as well as an understanding of virus transport and attenuation in aquatic systems. This proposed project will develop and improve methods for the enrichment and subsequent detection of human pathogenic viruses (e.g. adenoviruses, noroviruses, enteroviruses, rotaviruses) as well as bacteriophages (coliphages) in water samples. Moreover, as a first step towards molecular virus monitoring, it will test the potential of adenoviruses and noroviruses in two roles: (i) as putative indicator viruses reporting the presence of human pathogenic viruses and (ii) as reference viruses for investigating the efficiency of virus removal. The project will then combine the reality check of the new methods with investigations of viral transport and attenuation in waters and sediments in technical-scale experiments, many of which will be conducted in collaboration with partners from the consortium. Our own laboratory (batch and column) and semi-technical-scale experiments will address mechanisms responsible for virus mobilization and transport in the subsurface, with a focus on colloidal transport and the phenomenon of 'preferential flow'. By investigating keystone conditions, we aim to derive a set of parameters to determine a basis for estimating the risk of virus transport.

针对水中人类致病病毒的公共卫生保护需要更有效的病毒富集技术和灵敏的检测方法以及对水生系统中病毒传播和减毒的了解。该拟议项目将开发和改进水样中人类致病病毒（例如腺病毒、诺如病毒、肠道病毒、轮状病毒）以及噬菌体（大肠杆菌噬菌体）的富集和后续检测方法。此外，作为分子病毒监测的第一步，它将测试腺病毒和诺如病毒在两个角色中的潜力：（i）作为报告人类致病病毒存在的假定指示病毒；（ii）作为研究病毒清除效率的参考病毒。然后，该项目将把新方法的现实检验与技术规模实验中的病毒传播和水体及沉积物衰减研究结合起来，其中许多实验将与该联盟的合作伙伴合作进行。我们自己的实验室（批量和柱）和半技术规模的实验将解决病毒在地下动员和运输的机制，重点是胶体运输和“优先流动”现象。通过调查关键条件，我们的目标是得出一组参数，以确定估计病毒传播风险的基础。