Acquisition of a QUATTRO II Mass Spectrometer for the W.M. Keck Resource Laboratory at Yale University

在过去的几年里，在多肽测序的质谱仪的灵敏度、亲本离子扫描方法快速检测和识别大量蛋白质翻译后修饰的能力以及温和的电离技术将质谱学扩展到非共价蛋白质相互作用和折叠领域的前景方面取得了巨大的进步。这些技术传播得很慢，因为在这些新技术方面有过良好记录的实验室为数不多。目前的提议是通过购买QTOF质谱仪将最先进的质谱学带到耶鲁大学。该工具将支持目前没有可比工具的10个机构的研究。这些项目包括广泛的生物学研究领域，包括tRNA合成；前信使核糖核酸成熟；早期神经发生；细胞分化和生长的控制；细胞内膜运输；蛋白质结构和动力学；抗原受体多样性的产生；以及细胞表面糖基化在建立细胞特性中的作用。

Over the past few years there has been tremendous advances in the sensitivity of mass spectrometers for peptide sequencing, the ability of parent ion scanning approaches to rapidly detect and identify a plethora of protein posttranslational modifications and the promise of gentle ionization techniques to extend mass spectroscopy into the realm of non-covalent protein interactions and folding. These techniques have been disseminated slowly because of the small number of laboratories with demonstrated track record in these new techniques. The current proposal is intended to bring state-of-the-art mass spectroscopy to Yale University through the purchase of a QTOF mass spectrometer. This instrument will support research in 10 institutions that do not currently have a comparable instrument. The projects include a broad spectrum of biological research areas including tRNA synthesis; pre- mRNA maturation; early neurogenesis; the control of cellular differentiation and growth; intracellular membrane transport; protein structure and dynamics; the generation of antigen receptor diversity; and the role of cell surface glycosylation in establishing cellular identity.