NANO ELECTROSPRAY ON QUATTRO II TANDEM QUADRUPOLE MASS SPECTROMETER

Micromass Quattro II的电喷雾电离源 串联四极杆质谱仪已经被构造用于与 低流速（nL/min），与Mann描述的相当 等作为纳米喷雾剂。 该系统包括两个用于定位的摄像头 针头（尖端）和观察流量。 因为 Quattro II仪器的离子源外壳限制了 进入这个地区，之前必须测试几种设计， 达到了可接受的性能。 氮气管线加热器 还安装并测试了与水和其他较少的 挥发性溶剂 该系统已用于ESI-MS 和天然和衍生寡糖的ESI MS/MS分析， 糖缀合物、肽和蛋白质。

An electrospray ionization source for the Micromass Quattro II tandem quadrupole mass spectrometer has been constructed for use with low flow rates (nL/min), comparable to what has been described by Mann et al. as nanospray. The system includes two cameras for positioning the needle (tip) and observation of the flow. Because the geometry of the ion source housing of the Quattro II instrument has restricted access to this region, several designs had to be tested before acceptable performance was achieved. A heater for the nitrogen line also was installed and tested for use with aqueous and other less volatile solvents. The system has so far been utilized for the ESI-MS and ESI MS/MS analysis of native and derivatized oligosaccharides and glycoconjugates, peptides and proteins.