Acquisition of a Fluorescence Microplate Reader

获取荧光酶标仪

• 批准号: 9871266
• 负责人: Duane McPherson
• 金额: $ 2.47万
• 依托单位: SUNY College at Geneseo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9871266&HistoricalAwards=false
• 关键词: Acquisition; Fluorescence; Microplate; Reader

06/16/98 Recent advances in instrumentation now enable the use of fluorescence techniques for applications that previously required radioactive isotopes. Specific application examples include cell proliferation assays, assays for intracellular second messengers such a cyclic adenosine monophosphate (cAMP), and dynamic measurement of intracellular calcium activity. The principal investigators propose to purchase a CytoFluor Series 4000 multi-well fluorescence microplate reader to reduce the need for radioisotopes in teaching and research, to train students in the application of up-to-date fluorescence-based techniques, and to enhance student and faculty research in the department of Biology. The projects to be supported include research investigating immunology, neurobiology, invertebrate reproduction, and microbiology.

06/16/98 仪器的最新进展现在使荧光技术能够用于以前需要放射性同位素的应用。 具体应用实例包括细胞增殖测定、细胞内第二信使如环腺苷一磷酸（cAMP）的测定和细胞内钙活性的动态测量。 主要研究人员建议购买CytoFluor系列4000多孔荧光酶标仪，以减少教学和研究中对放射性同位素的需求，培训学生应用最新的基于荧光的技术，并加强生物系的学生和教师的研究。 支持的项目包括免疫学、神经生物学、无脊椎动物生殖和微生物学的研究。