Acquisition of a Fluorescence Microplate Reader

06/16/98仪器仪表的最新进展现在使荧光技术能够用于以前需要放射性同位素的应用。具体的应用实例包括细胞增殖分析、细胞内第二信使的分析，如环磷酸腺苷(CAMP)，以及细胞内钙活性的动态测量。首席研究人员建议购买一台CytoFluor Series 4000多孔荧光微孔板阅读器，以减少教学和研究中对放射性同位素的需求，培训学生应用最新的基于荧光的技术，并加强生物系的学生和教员的研究。将得到支持的项目包括研究免疫学、神经生物学、无脊椎动物繁殖和微生物学。

06/16/98 Recent advances in instrumentation now enable the use of fluorescence techniques for applications that previously required radioactive isotopes. Specific application examples include cell proliferation assays, assays for intracellular second messengers such a cyclic adenosine monophosphate (cAMP), and dynamic measurement of intracellular calcium activity. The principal investigators propose to purchase a CytoFluor Series 4000 multi-well fluorescence microplate reader to reduce the need for radioisotopes in teaching and research, to train students in the application of up-to-date fluorescence-based techniques, and to enhance student and faculty research in the department of Biology. The projects to be supported include research investigating immunology, neurobiology, invertebrate reproduction, and microbiology.