Collections Improvement for Genetic Resources at the Burke Museum

伯克博物馆遗传资源藏品的改进

• 批准号: 9876809
• 负责人: Scott Edwards
• 金额: $ 7.88万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-15 至 2001-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9876809&HistoricalAwards=false
• 关键词: Collections; Improvement; Genetic; Resources; Burke

With about 14,000 vertebrate individuals represented in its holdings (mostly birds), the Burke Museum Genetic Resources Collection at the University of Washington (UW)is among the top five such collections for birds in the United States and is actively growing at a rate of about 1,500 individual vertebrate representatives per year. The Collection is surprisingly broad for its size, with recent major acquisitions of frozen tissue specimens from northern Asia, Nicaragua, South Africa, Argentina, Australia, the Solomon Islands and the North Pacific Ocean. Two of the most valuable attributes of the collection are its focus on general, as opposed to research-driven, collecting, and its near complete representation of individual tissue specimens as voucher and extended wing specimens in the Ornithology and Mammalogy Collections. The increasing momentum of the Genetic Resources Collection is evident in several recent appointments, including a Curator of Genetic Resources, a Curator of Mammals, and a Manager of Genetic Resources.In the last five years, the Collection has distributed over 1,700 samples to systematists, evolutionary biologists and biomedical scientists in 8 countries at a rate that continues to grow. The Genetic Resources Collection has provided samples that have contributed to over 35 publications in the last five years, and in several cases has provided the core materials for doctoral dissertations both at the University of Washington and elsewhere. The Collection is curated by a Curator of Genetic Resources and a full-time Manager of Genetic Resources. Nonetheless, increasing demands on specimen storage and Curation of the collection, due both to increased specimen acquisition from University and privately funded field expeditions and to increased demand for specimens from inside and outside the UW, necessitate funding for further storage equipment, computers and labor to reduce the backlog of specimens not yet integrated into the Collection and database.To meet this increase in demand, this project will assist in the (1) purchase two additional upright -80C freezers and associated CO 2 backup systems, racks, storage boxes, specimen tubes and electrical modifications; (2) purchase an improved database backup system; (3) employ a graduate student to transfer a backlog of 1,500 tissues stored in large vials of lysis buffer and alcohol to 2 ml tubes for freezing; to inventory, transfer to 2 ml vials, install and enter data for 550 heterogeneous frozen tissues not yet inventoried; and to print and affix computer generated labels to all tubes in the collection; (4) purchase anice maker and dry ice storage vessel for superior storage of tissues in the field and for streamlining the tissue loan protocol; (5) purchase a computer and monitor dedicated to the Genetic Resources Database. The availability of extra freezers, dry ice storage, and an ice maker will increase the quality of tissues when transferred from local field sites and the ease with which tissues from recently returned expeditions can be organized and accessioned. The computer will increase self-sufficiency of the Curator and Manager in curation and management of its database.

它的藏品中有大约14,000只脊椎动物(主要是鸟类)，华盛顿大学(UW)的伯克博物馆遗传资源收藏是美国此类鸟类收藏的前五名之一，并以每年约1,500只脊椎动物代表的速度活跃增长。该藏品的规模之广令人惊讶，最近从北亚、尼加拉瓜、南非、阿根廷、澳大利亚、所罗门群岛和北太平洋获得了大量冷冻组织标本。该收藏的两个最有价值的属性是，它专注于一般的收集，而不是研究驱动的收集，以及在鸟类学和哺乳动物学收藏中将单个组织标本近乎完整地表示为凭证和扩展的翅膀标本。遗传资源收藏的增长势头从最近的几项任命中可见一斑，其中包括一名遗传资源馆长、一名哺乳动物馆长和一名遗传资源管理员。在过去的五年中，该馆藏向8个国家的系统学家、进化生物学家和生物医学科学家分发了1700多个样本，而且还在继续增长。遗传资源库在过去五年中为35种以上的出版物提供了样本，并在几个案例中为华盛顿大学和其他地方的博士论文提供了核心材料。该藏品由一名遗传资源馆长和一名全职遗传资源经理管理。然而，由于从大学和私人资助的实地考察获得的标本越来越多，以及对来自威斯康星州大学内外的标本的需求增加，对标本存储和保存的需求不断增加，有必要为更多的存储设备、计算机和劳动力提供资金，以减少尚未整合到收集和数据库中的标本的积压。为了满足这种需求的增加，该项目将协助(1)购买两个额外的直立式-80C冷冻机和相关的二氧化碳备份系统、机架、储存箱、标本管和电子改装；(2)购买改进的数据库备份系统；(3)雇用一名研究生将积压的1,500个储存在大瓶裂解缓冲液和酒精中的组织转移到2毫升试管中冷冻；清点、转移到2毫升试管中，安装和输入550个尚未编目的异种冷冻组织的数据；并打印计算机生成的标签，并将计算机生成的标签贴在收藏的所有试管上；(4)购买Anice Maker和干冰储存容器，用于现场更好地储存组织和简化组织贷款程序；(5)购买一台专门用于遗传资源数据库的计算机和监视器。额外的冰柜、干冰存储和制冰机的可用将提高从当地野外地点转移的组织的质量，并使最近返回的探险队的组织可以更容易地组织和获取。该计算机将提高馆长和管理员在管理和管理其数据库方面的自给自足能力。